This product is provided as a solution with a minimum concentration of 0.50 mg protein/mL Carboxypeptidase B in 0.05 M NaOAc pH 5.0, containing 1.0 M NaCl and 0.01% NaN3. The concentration is lot-specific and reported in the product Certificate of Analysis. The maximum volume, based on the product specification, is 0.2 mL. This product is stored at −20°C and ships on dry ice. Please see the link below to review a sample or lot-specific Certificate of Analysis:
https://www.sigmaaldrich.com/product/sigma/c5233#product-information
C5233
Carboxypeptidase B from human pancreas
50-55 units/mg protein carboxypeptidase B
Synonym(s):
Peptidyl-L-Lysine[L-arginine] hydrolase
Sign In to View Organizational & Contract Pricing.
Select a Size
100 μG
$2,280.00
$2,280.00
Please contact Customer Service for Availability
Skip To
Product Name
Carboxypeptidase B from human pancreas, 50-55 units/mg protein carboxypeptidase B
SMILES string
S(Cc3ccccc3)C(=O)C(NC(=O)C2N(CCC2)C(=O)C(NC(=O)C(NC(=O)CCC(=O)O)C)C)Cc1ccccc1
InChI key
TWURVFFNODFJBJ-UHFFFAOYSA-N
InChI
1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
biological source
human pancreas
form
solution
specific activity
50-55 units/mg protein carboxypeptidase B
impurities
≤0.2% chymotrypsin
≤0.2% trypsin
≤1 unit/mg protein carboxypeptidase A
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
human ... CPB1(1360)
Related Categories
1 of 4
This Item | C9584 | 217356 | C8946 |
|---|---|---|---|
| biological source human pancreas | biological source Porcine pancreas | biological source Porcine pancreas | biological source human pancreas |
| Gene Information human ... CPB1(1360) | Gene Information - | Gene Information - | Gene Information human ... CTRB1(1504) |
| specific activity 50-55 units/mg protein carboxypeptidase B | specific activity ≥125 units/mg protein | specific activity ≥100 units/mg protein | specific activity - |
| form solution | form lyophilized powder | form lyophilized | form lyophilized powder |
| shipped in dry ice | shipped in - | shipped in ambient | shipped in - |
| UniProt accession no. | UniProt accession no. - | UniProt accession no. - | UniProt accession no. |
Application
Carboxypeptidase B from Sigma has been used as a reference for assaying carboxypeptidase activity in lysed pituitary granules derived from the anterior and intermediate lobes of rat.[1] The enzyme has also been used to digest plasma samples by removing C-terminal basic amino acids, to get a distinct band for each allotype during C4 electrophoresis.[2]
Biochem/physiol Actions
Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 34,000 Da. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro.[3] The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.
General description
Other Notes
One unit will hydrolyze 1 μmole of hippuryl-L-arginine per minute at pH 7.7 at 25 °C
Physical form
Solution in 0.05 M NaOAc pH 5.0 + 1.0 M NaCl + 0.01% NaN3
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
E Sim et al.
The Biochemical journal, 239(3), 763-767 (1986-11-01)
The plasma complement protein C4 is encoded at two highly polymorphic loci, A and B, within the class-III region of the major histocompatibility complex. At least 34 different polymorphic variants of human C4 have been identified, including non-expressed or 'null'
Xiu Qin Xu et al.
The Journal of biological chemistry, 280(25), 23987-24003 (2005-04-23)
We have exploited a discrepancy in the oncogenic potential of autocrine and exogenous human growth hormone (hGH) in an attempt to identify molecules that could potentially be involved in oncogenic transformation of the human mammary epithelial cell. Microarray analysis of
Lawrence L K Leung et al.
Advances in experimental medicine and biology, 632, 61-69 (2008-11-26)
Thrombin-activatable procarboxypeptidase B (proCPB or thrombin-activatable fibrinolysis inhibitor or TAFI) is a plasma procarboxypeptidase that is activated by the thrombin-thrombomodulin complex on the vascular endothelial surface. The activated CPB removes the newly exposed carboxyl terminal lysines in the partially digested
J W Brodrick et al.
Biochimica et biophysica acta, 452(2), 468-481 (1976-12-08)
Carboxypeptidase B (peptidyl-L-lysine (-L-arginine) hydrolase, EC 3.4.12.3) has been isolated and purified to apparent homogeneity from activated extracts of human pancreas tissue. The purified enzyme has been shown to be a single polypeptide of 34 000 daltons. In this respect
Saurabh Chatterjee et al.
Free radical biology & medicine, 46(4), 454-461 (2008-12-04)
Post-translational modification of proteins due to exposure to radicals and other reactive species are markers of metabolic and inflammatory oxidative stress such as sepsis. This study uses the nitrone spin-trap DMPO and a combination of immuno-spin trapping and mass spectrometry
Related Content
QC Methods
-
I have a question about the Carboxypeptidase B (C-5233). Generally, how much volume of buffer solution (0.05M NaOAc pH 5.0+1.0M NaCl+0.01% NaN3) to add to make the stock? What is the storage temp. for the stock solution after reconstitution? Thank you!
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service