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C-12001

Normal Human Epidermal Keratinocytes (NHEK)

Juvenile foreskin, single donor, 500,000 cryopreserved cells

Synonym(s):

NHEK cells

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$762.00

About This Item

NACRES:
NA.81
UNSPSC Code:
41106514
Biological source:
human foreskin (juvenile epidermis)
Morphology:
( epidermal)

$762.00

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biological source

human foreskin (juvenile epidermis)

packaging

pkg of 500,000 cells

morphology

( epidermal)

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

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Normal Human Epidermal Keratinocytes (NHEK) Juvenile foreskin, single donor, 500,000 cryopreserved cells

Sigma-Aldrich

C-12001

Normal Human Epidermal Keratinocytes (NHEK)

Normal Human Epidermal Keratinocytes (NHEK) Adult, single donor, 500,000 cryopreserved cells

Sigma-Aldrich

C-12003

Normal Human Epidermal Keratinocytes (NHEK)

Normal Human Epidermal Keratinocytes (NHEK) Adult, single donor, 1 million cells per pellet

Sigma-Aldrich

C-14002

Normal Human Epidermal Keratinocytes (NHEK)

Normal Human Epidermal Keratinocytes (NHEK) Juvenile foreskin, pooled, 500,000 cryopreserved cells

Sigma-Aldrich

C-12005

Normal Human Epidermal Keratinocytes (NHEK)

morphology

( epidermal)

morphology

( epidermal)

morphology

(epidermal)

morphology

( epidermal)

biological source

human foreskin (juvenile epidermis)

biological source

human epidermis

biological source

human epidermis (adult)

biological source

human foreskin (juvenile epidermis)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−20°C

storage temp.

−196°C

packaging

pkg of 500,000 cells

packaging

pkg of 500,000 cells

packaging

pkg of 1,000,000 cells

packaging

pkg of 500,000 cells

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Application

Primary Normal Human Epidermal Keratinocytes (NHEK) are isolated from the epidermis of juvenile foreskin from a single donor and are provided in a cryopreserved format. They are the major cell type in the epidermis, making up about 90% of the cells. Epidermal keratinocytes originate in the stratum basale and move up through the layers of the epidermis. During this movement, they undergo gradual differentiation and morphology changes until they reach the stratum corneum, where they form a layer of nucleus-free, flat, and highly keratinized squamous cells. This layer forms an effective barrier to the entry of infectious agents into the body and minimizes moisture loss. Keratinocytes are also able to produce a variety of cytokines, growth factors, interleukins and complement factors. Therefore keratinocytes are important for wound healing, inflammation, and immune response.Normal Human Dermal Fibroblasts (NHDF) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available on request.

Biochem/physiol Actions

Skin

Preparation Note

Subculture Routine-Click here for more information.

Analysis Note

Rigid quality control tests are performed for each lot of Normal Human Epidermal Keratinocytes. They are tested for cell morphology, adherence rate, and cell viability. Furthermore, immunohistochemical tests for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 5000 cells per cm2Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: >15
Recommended Primary Cell Culture Media:Link

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Articles

Primary Human Epidermal Keratinocytes Cell Culture System

Primary human keratinocytes and serum-free keratinocyte growth media for the feeder cell-free in vitro cultivation of epidermal keratinocytes. Protocols for handling, cell passaging, media, and product use.

Protocols

Generating Organotypic Air-Liquid Interface (ALI) 3D Skin Cultures as an In-Vitro Model of the Human Epidermis

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

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