Skip to Content
MilliporeSigma
Get 22% off for Pi Day through March 31.Save Now

Key Documents

View All Documentation

Skip To

B6429

Casein Blocking Buffer 10x

for Northern and Southern blotting, solution

Synonym(s):

10x Blocking Buffer

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice

About This Item

NACRES:
NA.54
UNSPSC Code:
12161700
Pricing and availability is not currently available.
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

grade

Molecular Biology

Quality Level

200

form

solution

technique(s)

Southern blotting: suitable

storage temp.

room temp

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
C7594G766393017
Casein Blocking Buffer 10x for Northern and Southern blotting, solution

Sigma-Aldrich

B6429

Casein Blocking Buffer 10x

Casein Blocking Buffer for Northern and Southern blotting, powder blend

Sigma-Aldrich

C7594

Casein Blocking Buffer

Gelatin blocking buffer for Western blotting, powder blend

Sigma-Aldrich

G7663

Gelatin blocking buffer

SSC Buffer 20x for Northern and Southern blotting, solution

Sigma-Aldrich

93017

SSC Buffer 20x

technique(s)

Southern blotting: suitable

technique(s)

Southern blotting: suitable

technique(s)

-

technique(s)

Southern blotting: suitable

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

form

solution

form

powder blend

form

powder blend

form

solution

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

General description

Casein is a phosphoprotein found in milk. It is classified into four subtypes, including αS1-, αS2-, β-, and κ-caseins.[1]
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents[1] to the Northern and Southern Blot membranes.

Application

Casein Blocking Buffer 10x has been used in:

  • western blotting[2][3][4]
  • enzyme linked immunosorbent assay (ELISA)
  • immunohistochemistry

Features and Benefits

  • Better blocking performance than dried milk or BSA
  • Easy-to-use concentrate
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent and colorimetric detection assays

Preparation Note

Dilute 10x Blocking Buffer with molecular biology grade water before use.

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000531163
0000531163
0000500147
0000500147
0000484834

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Choline availability modulates human neuroblastoma cell proliferation and alters the methylation of the promoter region of the cyclin-dependent kinase inhibitor 3 gene
Niculescu MD, et al.
Journal of Neurochemistry, 89(5), 1252-1259 (2004)
Tanja Kalic et al.
The journal of allergy and clinical immunology. In practice, 8(9), 3084-3092 (2020-05-12)
Fish collagen is widely used in medicine, cosmetics, and the food industry. However, its clinical relevance as an allergen is not fully appreciated. This is likely due to collagen insolubility in neutral aqueous solutions, leading to low abundance in commercially
Mihai D Niculescu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 20(1), 43-49 (2006-01-06)
The availability of choline during critical periods of fetal development alters hippocampal development and affects memory function throughout life. Choline deficiency during fetal development reduces proliferation and migration of neuronal precursor cells in the mouse fetal hippocampus and these changes
M B Engelund et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 302(2), R300-R311 (2011-10-07)
Claudins are the major determinants of paracellular epithelial permeability in multicellular organisms. In Atlantic salmon (Salmo salar L.), we previously found that mRNA expression of the abundant gill-specific claudin 30 decreases during seawater (SW) acclimation, suggesting that this claudin is
Anna Ah-San Tang et al.
Science signaling, 10(505) (2017-11-16)
Some 30 years after its discovery, phage display remains one of the most widely used methods of in vitro selection. Initially developed to revolutionize the generation of therapeutic antibodies, phage display is now the first choice for screening artificial binding
View All Related Papers

Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Global Trade Item Number

SKUGTIN
B6429-500ML04061833434796

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service