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Amido Black Staining Solution 2X

Name: Amido Black Staining Solution 2<SC>X</SC>
Brand: SIGMA
Price: 141 USD
Availability: InStock

electrophoresis reagent

Synonym(s):

Naphthol Blue Black solution, Amido Black 10B

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Pack Size	SKU	Availability	Price
1 ea		Available to ship TODAYfromMILWAUKEE	$141.00

About This Item

Empirical Formula (Hill Notation):

C₂₂H₁₄N₆Na₂O₉S₂

CAS Number:

1064-48-8

Molecular Weight:

616.49

UNSPSC Code:

12171500

NACRES:

NA.32

PubChem Substance ID:

24891210

MDL number:

MFCD00004017

Beilstein/REAXYS Number:

5374263

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Quality Level

200

form

liquid

SMILES string

[Na+].[Na+].Nc1c(\N=N/c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\C=C/c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;

InChI key

AOMZHDJXSYHPKS-DROYEMJCSA-L

Related Categories

Clinical & Diagnostics

Dry Dyes & Certified Stains

Tissue Diagnostics

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Show Differences

1 of 1

This Item	N3393	N9002	199664
Sigma-Aldrich A8181 Amido Black Staining Solution 2X	Sigma-Aldrich N3393 Naphthol Blue Black	Sigma-Aldrich N9002 Naphthol Blue Black	Sigma-Aldrich 199664 Sudan Black B
form liquid	form powder	form powder	form powder
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

General description

Amido black (AB) is an acidic dye used for staining proteins. It can detect proteins levels of 50ng in both polyvinylidene difluoride (PVDF) and nitrocellulose membrane. AB aids protein visualization even at low concentrations. It has applications in forensics due to its ability to stain blood proteins in fingerprints.

Application

Amido Black Staining Solution 2X has been used in the staining:

serum proteins present in the culture supernatant[1]
cysteine sulfinic acid decarboxylase protein in mouse liver homogenate
histones from chromatin samples

Preparation Note

After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid.

related product

Product No.

Description

Pricing

G1041

EZBlue^™ Gel Staining Reagent

pictograms

GHS02,GHS07

signalword

Danger

hcodes

H225,H315,H319,H336

pcodes

P210 - P233 - P240 - P241 - P303 + P361 + P353 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Central nervous system

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

53.6 °F

flash_point_c

12 °C

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Impairments in left ventricular mitochondrial bioenergetics precede overt cardiac dysfunction and remodelling in Duchenne muscular dystrophy.

Meghan C Hughes et al.

The Journal of physiology, 598(7), 1377-1392 (2019-01-24)

Ninety-eight per cent of patients with Duchenne muscular dystrophy (DMD) develop cardiomyopathy, with 40% developing heart failure. While increased propensity for mitochondrial induction of cell death has been observed in left ventricle, it remains unknown whether this is linked to

Protein Blotting and detection: methods and protocols, 86(1), 10-18 (2009)

Placental release of taurine to both the maternal and fetal circulations in human term pregnancies

Holm MB, et al.

Amino Acids, 50(9), 1205-1214 (2018)

Detection of proteins on blot membranes

Goldman A, et al.

Current Protocols in Protein Science / Editorial Board, John E. Coligan ... [Et Al.], 86(1), 10-18 (2016)

Adsorptive Behavior of Kaolin for Amido Black Dye in Aqueous Solution

Ayanda OS, et al.

Orient. J. Chem., 34(3), 1233-1239 null

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Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Global Trade Item Number

SKU	GTIN
A8181-1EA	04061838251619

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