Key Documents

Anti-β-Actin (ACTB) Antibody

Name: Anti-β-Actin (ACTB) Antibody
Brand: SIGMA
Price: 580 USD
Availability: InStock

mouse monoclonal, AC-74

Synonym(s):

Actin Antibody Sigma, Beta Actin Antibody, Beta Actin Antibody - Monoclonal Anti-β-Actin antibody produced in mouse, Beta Actin Antibody Sigma, Sigma Beta Actin Antibody

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Pack Size	Availability	Price
100 μL	Available to ship onMarch 24, 2026fromMILWAUKEE	$580.00
0.2 mL	Available to ship onMarch 24, 2026fromMILWAUKEE	$735.00
0.5 mL	Available to ship onMarch 24, 2026fromMILWAUKEE	$1,430.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00164531

Conjugate:

unconjugated

Clone:

AC-74, monoclonal

Application:

ELISA (i), IF, IHC (p), WB

Citations:

4090

$580.00

Available to ship onMarch 24, 2026Details

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Product Name

Monoclonal Anti-β-Actin antibody produced in mouse, clone AC-74, ascites fluid

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

AC-74, monoclonal

mol wt

antigen 42 kDa

contains

15 mM sodium azide

species reactivity

rabbit, sheep, cat, guinea pig, bovine, mouse, chicken, wide range, Hirudo medicinalis, canine, Drosophila, carp, pig, rat, human

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This Item	A5441	A3854	A4700
Sigma-Aldrich A5316 Monoclonal Anti-β-Actin antibody produced in mouse	Sigma-Aldrich A5441 Monoclonal Anti-β-Actin antibody produced in mouse	Sigma-Aldrich A3854 Anti-β-Actin−Peroxidase antibody, Mouse monoclonal	Sigma-Aldrich A4700 Monoclonal Anti-Actin antibody produced in mouse
antibody form ascites fluid	antibody form ascites fluid	antibody form purified immunoglobulin	antibody form ascites fluid
species reactivity rabbit, sheep, cat, guinea pig, bovine, mouse, chicken, wide range, Hirudo medicinalis, canine, Drosophila, carp, pig, rat, human	species reactivity sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig	species reactivity sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig	species reactivity human, Xenopus, hamster, mouse, guinea pig, rat, rabbit, snail, sheep, canine, carp, chicken, pig, bovine, viper, goat
clone AC-74, monoclonal	clone AC-15, monoclonal	clone AC-15, monoclonal	clone AC-40, monoclonal
biological source mouse	biological source mouse	biological source mouse	biological source mouse
conjugate unconjugated	conjugate unconjugated	conjugate peroxidase conjugate	conjugate unconjugated
Gene Information human ... ACTB(60) mouse ... Actb(11461) rat ... Actb(81822)	Gene Information human ... ACTB(60) mouse ... Actb(11461) rat ... Actb(81822)	Gene Information human ... ACTB(60) mouse ... Actb(11461) rat ... Actb(81822)	Gene Information human ... ACTA1(58), ACTA1(58), ACTA1(58), ACTA2(59), ACTA2(59), ACTA2(59), ACTB(60), ACTC1(70), ACTC1(70), ACTC1(70), ACTG1(71), ACTG1(71), ACTG1(71), ACTG2(72), ACTG2(72), ACTG2(72) mouse ... Acta1(11459), Acta1(11459), Acta1(11459), Acta2(11475), Acta2(11475), Acta2(11475), Actb(11461), Actb(11461), Actb(11461), Actc1(11464), Actc1(11464), Actc1(11464), Actg1(11465), Actg1(11465), Actg1(11465), Actg2(11468), Actg2(11468), Actg2(11468) rat ... Acta1(29437), Acta1(29437), Acta1(29437), Acta2(81633), Acta2(81633), Acta2(81633), Actb(81822), Actb(81822), Actb(81822), Actc1(29275), Actc1(29275), Actc1(29275), Actg1(287876), Actg1(287876), Actg1(287876), Actg2(25365), Actg2(25365), Actg2(25365)

General description

Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle). ACTB (actin β) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. The ACTB gene is located on human chromosome 7p22.1.
Monoclonal Anti-β-Actin (mouse IgG2a isotype) is derived from the AC-74 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Recognizes an epitope located on the N-terminal end of the β-isoform of actin. It specifically labels β-actin in a wide variety of tissues and species. In immunofluorescent staining of chicken gizzard ultrathin cryosections, the antibody labels the dense bodies, the longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. The antibody does not react with adult cardiac or skeletal muscle (besides traces due to contamination of the sample with non-muscle cells) or with β-actin expressing cells in Dictyostelium discoideum amoeba. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. B5, methacarn, ethanol or Bouin′s solutions may be also used as fixatives. The antibody can be used as probes for β-actin as an internal control in immunoblotting.

Immunogen

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Application

Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.

Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.
Mouse Monoclonal Anti-β-Actin antibody has been used for western blot analyses. The antibody can also be used for immunohistochemistry, indirect immunofluorescence (1:1,000) using cultured human or chicken fibroblasts, and indirect ELISA.
Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.
Monoclonal Anti-β-Actin antibody has been used in western blotting.

Mouse Monoclonal Anti-β-Actin antibody has been used for western blot analyses. The antibody can also be used for immunohistochemistry, indirect immunofluorescence (1:1,000) using cultured human or chicken fibroblasts, and indirect ELISA.

Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.

Biochem/physiol Actions

The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. β-Actin (ACTB) modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder. It recognizes an epitope located on the N-terminal end of the β-isoform of actin. It specifically labels β-actin in a wide variety of tissues and species. In immunofluorescent staining of chicken gizzard ultrathin cryosections, the antibody labels the dense bodies, the longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. The antibody does not react with adult cardiac or skeletal muscle (besides traces due to contamination of the sample with non-muscle cells) or with β-actin expressing cells in Dictyostelium discoideum amoeba. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. B5, methacarn, ethanol or Bouin′s solutions may be also used as fixatives. The antibody can be used as probes for β-actin as an internal control in immunoblotting.

Physical form

The product is provided as ascites fluid containing 0.1% sodium azide as a preservative.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000540019

0000508933

0000464992

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Functional thermogenic beige adipogenesis is inducible in human neck fat.

Lee P, et al.

International Journal of Obesity, 38(2), 170-170 (2014)

Actin isoform compartments in chicken gizzard smooth muscle cells.

North A J, et al.

Journal of Cell Science, 107, 445-455 (1994)

TAK733 attenuates adrenergic receptor-mediated cardiomyocyte hypertrophy via inhibiting ErkThr188 phosphorylation.

Chang Youn Lee et al.

Clinical hemorheology and microcirculation, 72(2), 179-187 (2019-02-05)

Cardiac hypertrophy is an important risk factor for heart failure. The MEK-ERK axis has been reported as a major regulator in controlling cardiac hypertrophy. TAK733 is a potent and selective MEK inhibitor that suppresses cell growth in a broad range

Heparin-binding epidermal growth factor-like growth factor links hepatocyte priming with cell cycle progression during liver regeneration.

Mitchell C, et al.

The Journal of Biological Chemistry, 280(4), 2562-2568 (2005)

Myc confers androgen-independent prostate cancer cell growth.

Bernard D, et al.

The Journal of Clinical Investigation, 112(11), 1724-1731 (2003)

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Global Trade Item Number

SKU	GTIN
A5316-100UL	04061833375754
A5316-.5ML	04061833375747
A5316-.2ML	04061833375730

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