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A4685

Luminol sodium salt

luminescent, ≥98% (titration), powder and chunks

Synonym(s):

3-Aminophthalhydrazide, 5-Amino-2,3-dihydro-1,4-phthalazinedione

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About This Item

Linear Formula:
C8H6N3O2Na
CAS Number:
Molecular Weight:
199.14
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
MDL number:
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Product Name

Luminol sodium salt,

assay

≥98% (titration)

Quality Level

form

powder and chunks

solubility

water: 50 mg/mL, clear to very slightly hazy, colorless to faint red

SMILES string

O=C1NN([Na])C(C2=CC=CC(N)=C21)=O

InChI

1S/C8H7N3O2.Na/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12;/h1-3H,(H4,9,10,11,12,13);/q;+1/p-1

InChI key

RVJVDCVIJCBUTH-UHFFFAOYSA-M

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This Item
A8511123072A8264
assay

≥98% (titration)

assay

≥97% (HPLC)

assay

97%

assay

≥98% (HPLC)

solubility

water: 50 mg/mL, clear to very slightly hazy, colorless to faint red

solubility

DMSO: 50 mg/mL, clear to slightly hazy

solubility

-

solubility

2 M NH4OH: 50 mg/mL, clear to slightly hazy

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

form

powder and chunks

form

powder or chunks

form

-

form

powder

Application

Luminol sodium salt has been used:

  • as a component of MEBSS buffer in a bioluminescence assay to detect peroxidase activity in whole blood samples[1]
  • to study protein interaction in whole-cell lysates by immunoprecipitation[2]
  • for in vitro reactive oxygen species (ROS) quantification in CD45+ and CD45− cells

Biochem/physiol Actions

Luminol is a redox-sensitive chemiluminescent compound.[3] It emits blue luminescence when the basic aqueous solution of luminol having oxygen atoms come in contact with oxidizing agents like potassium ferricyanide. It is highly stable and is applicable in various fields such as analytical chemistry, metallurgy, biochemistry, and forensics for detecting reactive species.[3] Luminol has the potential to detect defects in neutrophils ex vivo during oxidative metabolism defects such as chronic granulomatous disease and myeloperoxidase deficiency.[3]

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Chemiluminescence detection in analytical chemistry
Lancaster, J. Steven
Endeavour, 16, 194-194 (1992)
New Developments in Chemiluminescence Research
Gill, Steven, K.
Aldrichimica Acta, 16, 59-59 (1983)
Yantu Zhang et al.
Journal of chromatography. A, 1154(1-2), 260-268 (2007-04-24)
A novel method was developed for the simultaneous determination of glucocorticoid residues such as triamcinolone (TR), prednisolone (PR), hydrocortisone (HC), cortisone (CO), methylprednisolone (MP), dexamethasone (DE) and triamcinolone acetonide (TA) by high-performance liquid chromatography (HPLC) coupled with chemiluminescence (CL) detection.
Opioids prevent regeneration in adult mammals through inhibition of ROS production
Elodie L, et al.
Scientific Reports, 8(1) (2018)
Chemilunimescence of luminol: The chemcial reaction
White E H, et al.
ACS Chemical Biology, 86(5), 940-941 (1964)

Global Trade Item Number

SKUGTIN
A4685-25G04061833374948
A4685-5G04061833374955
A4685-1G04061833374931

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