Skip to Content
Merck
All Photos(3)

Documents

A3157

Sigma-Aldrich

Alcian Blue 8GX

Dye content, ≥50%, certified by the Biological Stain Commission, powder

Synonym(s):

Alcian Blue, Ingrain Blue 1

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

Empirical Formula (Hill Notation):
C56H68Cl4CuN16S4
CAS Number:
33864-99-2
Molecular Weight:
1298.86
Colour Index Number:
74240
EC Number:
251-705-7
MDL number:
MFCD00010720
UNSPSC Code:
12171500
PubChem Substance ID:
24890721
NACRES:
NA.47

product name

Alcian Blue 8GX, certified by the Biological Stain Commission

grade

certified by the Biological Stain Commission

Quality Level

200

form

powder

composition

Dye content, ≥50%

color

dark blue

solubility

water: 1 mg/mL, blue

εmax

195-400 at 617-630 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(\SCc1ccc2c3nc(nc4n5[Cu]n6c(nc7nc(nc5c8cc(CS\C(N(C)C)=[N+](\C)C)ccc48)c9ccc(CS\C(N(C)C)=[N+](\C)C)cc79)c%10ccc(CS\C(N(C)C)=[N+](\C)C)cc%10c6n3)c2c1)=[N+](/C)C

InChI

1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-57-45(37)62-50-43-27-35(31-75-55(69(9)10)70(11)12)19-23-39(43)47(59-50)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(60-52)63-51-42-26-34(18-22-38(42)46(58-51)61-49)30-74-54(67(5)6)68(7)8;;;;;/h17-28H,29-32H2,1-16H3;4*1H;/q+2;;;;;+2/p-4

InChI key

CKLBXIYTBHXJEH-UHFFFAOYSA-J

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

Alcian Blue 8GX has been used for the staining of sulfated proteoglycans in murine MPC (mesenchymal progenitor cell) nodule. It has been used for the staining of sulfated proteoglycans in bovine articular chondrocytes.

Biochem/physiol Actions

Alcian Blue 8GX is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby helping in the quantitative determination of glycosaminoglycans. It is also used for the staining of bone and cartilage.

Suitability

Certified for use in staining in Mowry′s pH 2.5 method, in Scott′s pH 5.7 method, and in Kreyberg′s method for keratin and mucin.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Isolation of mouse marrow mesenchymal progenitors by a novel and reliable method.
Sun S, et al.
Stem Cells, 21, 527-535 (2003)
Chitosan/polyester-based scaffolds for cartilage tissue engineering: assessment of extracellular matrix formation.
Alves da Silva ML, et al.
Acta Biomaterialia, 6, 1149-1157 (2010)
Virginie Tissières et al.
Cell reports, 31(1), 107490-107490 (2020-04-09)
Digit loss/reductions are evolutionary adaptations in cursorial mammals such as pigs. To gain mechanistic insight into these processes, we performed a comparative molecular analysis of limb development in mouse and pig embryos, which revealed a loss of anterior-posterior polarity during
The quantitative determination of glycosaminoglycans in urine with Alcian Blue 8GX.
Whiteman P
The Biochemical Journal, 131, 351-357 (1973)
Lara Vlietstra et al.
PloS one, 14(6), e0217462-e0217462 (2019-06-07)
To determine if there is an association between sarcopenia, physical function and self-reported fatigue in osteoarthritis (OA) and rheumatoid arthritis (RA). A cross-sectional analysis of measurements from a cohort of 157 participants with OA or RA was performed. The relationship
View All Related Papers

Articles

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service