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XNAPS

REDExtract-N-Amp Plant PCR Kit

sufficient for 10 extractions, sufficient for 10 amplifications

Synonym(s):

Plant direct PCR kit, Plant direct PCR kit with loading dye

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1 KIT

$47.40

$47.40


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About This Item

NACRES:
NA.55
UNSPSC Code:
41106303

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Quality Level

usage

sufficient for 10 amplifications, sufficient for 10 extractions, sufficient for 10 reactions

feature

dNTPs included, hotstart, Direct PCR

technique(s)

PCR: suitable

color

red

input

crude DNA

storage temp.

−20°C

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This Item
XNAPXNAPRXNAP2
technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

usage

sufficient for 10 amplifications

usage

sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions

usage

sufficient for 1000 amplifications, sufficient for 1000 extractions, sufficient for 1000 reactions

usage

sufficient for 100 amplifications, sufficient for 100 extractions, sufficient for 100 reactions

feature

dNTPs included

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

color

red

color

red

color

red

color

colorless

XNAP

XNAPE, XNAPR, XNAPS, microbialanalysis
microbialanalysis

XNAP, XNAPE, XNAPS, microbialanalysis
microbialanalysis

XNAP2E, XNAPR, microbialanalysis
microbialanalysis

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

The REDExtract-N-Amp Plant PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. A novel Extraction Solution eliminates the need for conventional freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction, column purification, or precipitation of DNA. The kit also includes a PCR ReadyMix, especially formulated for amplification directly from extract. The REDExtract-N-Amp PCR ReadyMix contains a dye that acts as a tracking dye and allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

Genomic DNA is extracted from 0.5 to 0.7 cm plant leaf disks that have been cut with a standard paper punch and simply incubated in Extraction Solution at 95 oC for 10 minutes. An equal volume of Dilution Solution is added to the extract to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and the REDExtract-N-Amp ReadyMix, included in the kit.
The REDExtract-N-Amp Plant PCR Kits enable the rapid detection of plant pathogens by direct PCR (polymerase chain reaction). These kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by direct PCR. The REDExtract-N-Amp PCR ReadyMix contains a dye that acts as a tracking dye and allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

Application

REDExtract-N-Amp Plant PCR Kit has been used to:
  • extract genomic DNA from mycelial lawns and leaves[1][2][3]
  • screen leaf tissues from putative transgenic potato plants[4]
  • perform polymerase chain reaction (PCR)[5][6][7][8]

Features and Benefits

  • Genomic DNA for PCR in less than 15 minutes
  • A PCR ReadyMix, specially formulated for amplification directly from extract
  • Hot Start antibody for highly specific PCR amplification of genomic DNA
  • Extract stable at 4 °C for at least 6 months
  • Single-step extraction of plant genomic DNA for PCR in less than 15 minutes
  • No freezing, mechanical disruption, organic extraction, column purification or precipitation required
  • Specially formulated PCR ReadyMix for use with extract
  • Hot Start antibody for highly specific PCR amplification of genomic DNA
  • REDExtract-N-Amp requires no loading buffers or tracking dyes required for gel analysis
  • Compatible with high-throughput requirements for genetic analysis of plants
  • Extract stable at 4 °C for at least 6 months

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.
Kits contain Dilution solution, Extraction solution, and REDExtract-N-Amp PCR Reaction Mix.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

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Description
Pricing

pictograms

Exclamation markEnvironment

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Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3


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Neil D Havis et al.
FEMS microbiology letters, 256(2), 217-223 (2006-02-28)
Ramularia collo-cygni is a barley pathogen of increasing importance in Northern and Central Europe, New Zealand and South America. Accurate visual and microscopic identification of the pathogen from diseased tissue is difficult. A nested PCR-based diagnostic test has been developed
Rowan S McKibbin et al.
Plant biotechnology journal, 4(4), 409-418 (2006-12-21)
Transgenic potato (Solanum tuberosum cv. Prairie) lines were produced over-expressing a sucrose non-fermenting-1-related protein kinase-1 gene (SnRK1) under the control of a patatin (tuber-specific) promoter. SnRK1 activity in the tubers of three independent transgenic lines was increased by 55%-167% compared
Lorena C Garcia et al.
International journal of systematic and evolutionary microbiology, 60(Pt 2), 331-337 (2009-08-05)
A novel actinomycete, designated strain GUI 15(T), isolated from the root nodules of a Pisum sativum plant was characterized taxonomically by using a polyphasic approach. The 16S rRNA gene sequence of strain GUI 15(T) showed highest similarity to Micromonospora pattaloongensis
José F Cobo-Díaz et al.
Systematic and applied microbiology, 37(3), 177-185 (2013-11-26)
Genista versicolor is an endemic legume from Sierra Nevada National Park which constitutes one of the UNESCO-recognized Biosphere Reserves. In the present study, a collection of strains nodulating this legume was analysed in characteristic soils of this ecosystem. Most strains
Martha E Trujillo et al.
The ISME journal, 4(10), 1265-1281 (2010-05-07)
Our current knowledge of plant-microbe interactions indicate that populations inhabiting a host plant are not restricted to a single microbial species but comprise several genera and species. No one knows if communities inside plants interact, and it has been speculated

Articles

Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

Protocols

REDExtract-N-Amp Plant PCR kit includes loading dye for easy gel loading after plant genomic DNA extraction.

Related Content

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Global Trade Item Number

SKUGTIN
XNAPS-1KT04061837549922

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