The conductivity of this water is not determined. Currently, there is no Molecular Biology grade water product available that is tested for conductivity.
W4502
Water
suitable for electrophoresis, PCR
Water
Synonym(s):
DNase-Free Water, Not DEPC Treated, Nuclease-Free Water, Protease-Free Water, RNase-Free Water
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 L | Available to ship TODAYfromMILWAUKEE | $86.00 | |
| 10 x 50 mL | Available to ship TODAYfromMILWAUKEE | $151.00 | |
| 6 x 1 L | Check Cart for Availability | $404.00 | |
| 10 L | Available to ship TODAYfromMILWAUKEE | $421.00 |
About This Item
Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
NACRES:
NA.31
PubChem Substance ID:
UNSPSC Code:
12191602
EC Number:
231-791-2
MDL number:
Beilstein/REAXYS Number:
2050024
Technique(s):
PCR: suitable, electrophoresis: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg
$86.00
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Product Name
Water, Nuclease-Free Water, for Molecular Biology
vapor density
<1 (vs air)
Quality Level
vapor pressure
3 mmHg
sterility
0.1 μm filtered
form
liquid
technique(s)
PCR: suitable, electrophoresis: suitable
refractive index
n20/D 1.34 (lit.)
pH
5-7
bp
100 °C (lit.)
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1 of 1
This Item | |||
|---|---|---|---|
| technique(s) PCR: suitable, electrophoresis: suitable | technique(s) - | technique(s) - | technique(s) FISH: suitable |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| form liquid | form liquid | form liquid | form liquid |
| density 1.000 g/mL at 3.98 °C (lit.) | density 1.000 g/mL at 3.98 °C (lit.) | density 1.000 g/mL at 3.98 °C (lit.) | density 1.000 g/mL at 3.98 °C (lit.) |
| bp 100 °C (lit.) | bp 100 °C (lit.) | bp 100 °C (lit.) | bp 100 °C (lit.) |
| pH 5-7 | pH - | pH 5-7 | pH 5-7 |
Application
Water has been used-
- in cDNA synthesis and PCR amplification
- for the dilution of rumen DNA sample prior to qPCR assay
- in PCR (polymerase chain reaction) and agarose gel electrophoresis
- for the elution of RNA during RNA isolation
Features and Benefits
- Nuclease-Free Water
- DNase-Free, RNase-Free, Protease- Free
- Not DEPC Treated
- 0.1 um Filtered
- Prepared from Double Distilled and Deionized Water
- Suitable for use in Molecular Applications
This product is 0.1um filtered. It is designated as a Molecular Biology Reagent and is suitable for molecular biology applications. This product has been analyzed for the absence of nucleases and proteases, and has undergone bioburden analysis. It has not been treated with diethyl pyrocarbonate (DEPC).
Preparation Note
This product is prepared from double distilled and deionized water
Other Notes
Easily compare specifications for Water products with the Water specification table.
comparable product
Product No.
Description
Pricing
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Polymorphic exact tandem repeat A (PETRA): a newly defined lineage of mycobacterium tuberculosis in israel originating predominantly in Sub-Saharan Africa.
Freidlin PJ et al
Journal of Clinical Microbiology, 47(12), 4006-4020 (2009)
Comparison of coliforms and coliphages as tools for assessment of viral contamination in river water.
Skraber S et al
Applied and Environmental Microbiology, 70(6), 3644-3649 (2004)
Presence of viral genomes in mineral water: a sufficient condition to assume infectious risk?
Gassilloud B et al
Applied and Environmental Microbiology, 69(7), 3965-3969 (2003)
Effect of sward dry matter digestibility on methane production, ruminal fermentation, and microbial populations of zero-grazed beef cattle.
Hart KJ et al
Journal of Animal Science, 87(10), 3342-3350 (2009)
Gene expression signals involved in ischemic injury, extracellular matrix composition and fibrosis defined by global mRNA profiling of the human left ventricular myocardium.
Gabrielsen A et al
Journal of Molecular and Cellular Cardiology, 42(4), 870-883 (2007)
Articles
Nuclease-free water (or PCR water) is needed in molecular biology to avoid sample degradation during PCR, sequencing, etc. Ultrafiltration provides a quicker, safer, and more sustainable alternative to DEPC treatment when preparing RNase-free and DNase-free water.
Protocols
GenomePlex® Whole Genome Amplification efficiently extracts DNA from animal samples for genomic analysis.
Protocol extracts genomic DNA from blood cards, useful for limited DNA samples in amplification.
Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Related Content
KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield
Global Trade Item Number
| SKU | GTIN |
|---|---|
| W4502-1.5ML | 04061837774577 |
| W4502-100ML | 04061837774591 |
| W4502-5ML | 04061837774744 |
| W4502-6X1L | 04061837899300 |
| W4502-1L | 04061837899294 |
| W4502-10ML | 04061837774614 |
| W4502-1ML | 04061837774645 |
| W4502-10L | 04061837899287 |
| W4502-10X50ML | 04061841755142 |
| W4502-25ML | 04061837774683 |
| W4502-50ML | 04061837774720 |
| W4502-6X1L-I | 04061837899317 |
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W4502 Millipore Water: what is the ohms of this water?
1 answer-
Helpful?
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Do you have stability data for W4502 for evaluating if Shelf Life can be extended?
1 answer-
This water product is not assigned an expiration or retest date. In the original sealed container the material is stable nearly indefinitely. Items that are not assigned an expiration date are warranteed for 1 year from the date of shipment.
Helpful?
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Is this water sterile? Can this be used for cell culture?
1 answer-
Product W4502 is not sterile, this product is prepared from Double Distilled and Deionized Water. This product is best suitable to use in molecular biology applications. See product W3500 for Sterile, Tissue Culture grade Water:
https://www.sigmaaldrich.com/product/sigma/w3500Helpful?
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In what vessel does this product come? A bottle, a bag with a spigot, etc.?
1 answer-
This product is packaged in square PET square bottles. To request a quote for larger volumes or specialized packaging, please submit a request at the link below:
https://www.sigmaaldrich.com/services/support/bulk-quotation-requestHelpful?
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This product does not list an expiration date on the label. Could someone provide an expiration for lot number BCCH7145?
1 answer-
This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services
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