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W3516

Sigma-Aldrich

Anti-WSTF (C-Terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-BAZ1B, Anti-Bromdomain Adjacent to Zn Finger Domain 1B, Anti-Williams Syndrome Transcription Factor, Anti-Williams-Beuren Syndrome Chromosome Region 9

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About This Item

MDL number:
MFCD08705535
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 175 kDa

species reactivity

human, mouse

technique(s)

immunoprecipitation (IP): 5-10 μg using from lysates of HEK293-T cells
indirect immunofluorescence: 10-20 μg/mL using paraformaldehyde-fixed NIH3T3 cells
microarray: suitable
western blot: 0.5-1 μg/mL using extracts of MCF-7 breast cancer cell line

UniProt accession no.

Q9UIG0

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... BAZ1B(9031)
mouse ... Baz1b(22385)
rat ... Baz1b(368002)

General description

Williams syndrome transcription factor (WSTF) belongs to the WAL/BAZ/WAC protein family, encoded by the bromodomain adjacent to zinc finger domain 1B (BAZ1B) gene. It is a non-secretory protein, which consists of a C-terminal bromodomain adjacent to a PHD (plant homeodomain) finger.

Immunogen

synthetic peptide corresponding to amino acids 1467-1483 of human WSTF, conjugated to KLH via an N-terminal added cysteine residue. The corresponding rat and mouse sequence differ from the immunizing sequence by three amino acids.

Application

Anti-WSTF (C-Terminal) antibody produced in rabbit has been used in western blotting and immunofluorescence.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Williams syndrome transcription factor (WSTF) is implicated in transcription, replication, chromatin remodeling and DNA damage response. It acts as a tyrosine protein kinase. Mutations in this gene are associated with Williams-Beuren syndrome. WSTF might function as an activator of gene expression of cytochrome P450 family 21 subfamily A member 2 (CYP21A2) and CYP19A1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

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WSTF promotes proliferation and invasion of lung cancer cells by inducing EMT via PI3K/Akt and IL-6/STAT3 signaling pathways
Meng J, et al.
Cellular Signalling, 28(11), 1673-1682 (2016)
KRASG12 mutant induces the release of the WSTF/NRG3 complex, and contributes to an oncogenic paracrine signaling pathway
Liu Y, et al.
Testing, 7(33), 53153-53153 (2016)
Williams syndrome transcription factor (WSTF) acts as an activator of estrogen receptor signaling in breast cancer cells and the effect can be abrogated by 1alpha, 25-dihydroxyvitamin D3
Lundqvist J, et al.
The Journal of Steroid Biochemistry and Molecular Biology, 177, 171-178 (2018)
Differences in embryo quality are associated with differences in oocyte composition: a proteomic study in inbred mice
Pfeiffer MJ, et al.
Proteomics, 15(4), 675-687 (2015)
Martin J Pfeiffer et al.
Proteomics, 15(4), 675-687 (2014-11-05)
Current models of early mouse development assign roles to stochastic processes and epigenetic regulation, which are considered to be as influential as the genetic differences that exist between strains of the species Mus musculus. The aim of this study was
View All Related Papers

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