U4757
Anti-Urocortin antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
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About This Item
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biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
rat, human
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using microwave-treated sections of human placental tissue
quantitative precipitin assay: 1:10,000 using 125I-Tyr0-Urocortin (rat)
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... UCN(7349)
rat ... Ucn(29151)
General description
Urocortin (UCN) is a neuropeptide, widely distributed in the central nervous system (CNS). Urocortin is derived from a larger precursor protein (pro-urocortin, also named corticotensin).
Urocortin (UCN) is encoded by the gene mapped to human chromosome 2p21–23. The encoded protein belongs to the corticotropin-releasing hormone (CRH) family. UCN is a 40 amino acid long peptide. It is widely expressed in the heart, lung, skin and immune cells.
Specificity
The antibody is evaluated for activity and specificity by radioimmunoassay (RIA) and immunohistochemistry. The antibody does not cross-react with human or rat CRF (6-33), sheep CRF, PACAP38, human ACTH, human ACTH (18-39), and sauvagine.
Immunogen
synthetic peptide corresponding to the C-terminal of human urocortin (amino acids 25-40 with N-terminally added lysine) conjugated to KLH. The immunogen sequence (human) corresponds to pro-urocortin (amino acids 105-120) and is identical in rat urocortin.
Application
Anti-Urocortin antibody produced in rabbit has been used in:
- radioimmunoassay (RIA)
- immunohistochemistry
- immunocytochemistry
Anti-urocortin antibody produced in rabbit has been used to evaluate the entry of urocortin peptide into the brain.
Biochem/physiol Actions
Urocortin (UCN) plays a vital role in inhibiting transforming growth factor β1 (TGFβ1) oncogenic signaling pathway. The encoded protein is implicated in systemic and peripheral stress-response systems. Mutations in UCN gene might increase the risk of susceptibility to obesity.
Urocortin has biological actions like corticotropin-releasing factor (CRF) such as autonomic and behavioral responses in mammalian species. It is a potent stimulator of adrenocorticotropic hormone (ACTH) secretion by corticotroph cells from the anterior pituitary in vitro and in vivo. In the central nervous system (CNS), urocortin acts to produce potent anxiogenic-like and appetite suppressing effects and peripherally has hypotensive effects.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Secretion of urocortin I by human glioblastoma cell lines, possibly via the constitutive pathway.
Ikeda K
Peptides, 63, 63-70 (2015)
Cynthia L Bethea et al.
Brain research, 1488, 24-37 (2012-10-06)
Communication between the serotonin system and the CRF system plays a pivotal role in the mediation of stress and stress reactivity. CRF appears to be inhibitory of serotonin neurotransmission through the CRF receptor type 1 (CRF-R1). Serotonin neurons also detect
Mutation Screening of the Urocortin Gene: Identification of New Single Nucleotide Polymorphisms and Association Studies with Obesity in French Caucasians
Delplanque J
The Journal of Clinical Endocrinology and Metabolism, 87, 867-869 (2002)
R L Sanchez et al.
Neuroscience, 171(3), 893-909 (2010-09-14)
A significant number of postmenopausal women report increased anxiety and vulnerability to stress, which has been linked to decreased secretion of ovarian steroids. Communication between the serotonin system and the corticotropin releasing factor (CRF) system determines stress sensitivity or resilience.
Paraventricular hypothalamic nucleus: axonal projections to the brainstem
Geerling JC, et al.
The Journal of Comparative Neurology, 518(9), 1460-1499 (2010)
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