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T7941

Sigma-Aldrich

Monoclonal Anti-β-Tubulin IV antibody produced in mouse

clone ONS.1A6, ascites fluid

Synonym(s):

Anti-DYT4, Anti-TUBB4, Anti-beta-5

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About This Item

MDL number:
MFCD02095891
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

ONS.1A6, monoclonal

mol wt

antigen ~55 kDa

species reactivity

hamster, chicken, human, bovine, rat, mouse

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:400 using cultured human breast adenocarcinoma cell line MCF-7

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBB4(10382)
mouse ... Tubb4(22153)
rat ... Tubb4(29213)

General description

β-Tubulin IV codes for β-tubulin 4A (TUBB4A). TUBB4A is expressed in the central nervous system by oligodendrocytes. It is located on human chromosome 19p13.3.
Microtubules are heterodimeric proteins that comprise of α- and β-tubulins. β-tubulins have four different isotypes in brain cells, ranging from β I to β IV . Anti-tubulin antibodies are useful to study the localization of tubulin proteins. Monoclonal Anti-β-Tubulin IV antibody is specific for epitope located in the C-terminal sequence of β-tubulin isotype IV in humans, bovines, hamsters, rats, mice and chickens. The product does not react with other tubulin isotypes.

Immunogen

synthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype IV, conjugated to BSA.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunofluorescence (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-β-Tubulin IV antibody has been used in immunostaining.
Monoclonal Anti-β-Tubulin IV antibody is suitable for use in immunohistochemistry (formalin-fixed, paraffin-embedded sections), indirect ELISA, microarray and western blot (1:400 using cultured human breast adenocarcinoma cell line MCF-7). The product may also be used for immunocytochemistry and immunofluorescence .

Biochem/physiol Actions

β-Tubulin IV creates the oligodendrocyte microtubule network, that helps in the transport of myelin proteins, lipids and RNA at the time of myelination. It participates in the generation of early fibrotic stages. TUBB4 plays a major role in the transport and proper localization of N-cadherin within the plasma membrane.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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R Renthal et al.
Cell motility and the cytoskeleton, 25(1), 19-29 (1993-01-01)
Four different isotypes of beta-tubulin are known to be expressed in mammalian brain. Monoclonal antibodies against beta II, beta III, and beta IV were used to characterize the beta-tubulin isotypes in two ciliated bovine tissues: non-motile sensory cilia of retinal
Tubulin beta 3 and 4 are involved in the generation of early fibrotic stages
Wawro M E, et al.
Cellular Signalling, 38(3), 26-38 (2017)
The many faces of TUBB4A mutations
Lohmann K and Klein C
Neurogenetics, 15(2), 81-81 (2014)
beta IV tubulin is selectively expressed by oligodendrocytes in the central nervous system
Terada N, et al.
Glia, 50(3), 212-222 (2005)
Shubing Zhang et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 242(6), 678-686 (2013-03-26)
The Notch signaling pathway plays complex roles in developing lungs, including regulation of proximodistal fates, airway cell specification and differentiation. However, the specific Notch-mediated signals involved in lung development remain unclear. Here we report that Jagged1 is expressed in a
View All Related Papers

Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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