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SAB4200008

Sigma-Aldrich

Anti-DVL3 (N-terminal) antibody produced in rabbit

enhanced validation

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Dishevelled-3

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~95 kDa

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.5 mg/mL

technique(s)

immunoprecipitation (IP): 2.5-5 μg using HEK-293T cell lysate overexpressing human DVL3
western blot: 0.25-0.5 μg/mL using HEK-293T cell lysates overexpressing human DVL3

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DVL3(1857)

General description

Dishevelled 3 (DVL3) is a phosphoprotein rich in serine and threonine. It is widely expressed in fetal tissues and specifically expressed in various adult tissues. DVL3 gene is mapped to human chromosome 3q27.1

Specificity

Anti-DVL3 (N-terminal) specifically recognizes human DVL3.

Application

Anti-DVL3 (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Dishevelled 3 (DVL3) participates in the wingless-related integration site (Wnt) signaling pathway. It plays a key role in hippocampal neurogenesis regulation. Knockdown of DVL3 has been shown to attenuate the activation of the wnt signaling pathway by Wnt3A. DVL3 functions in redundant pathways with DVL1 and DVL2, and is necessary in normal cardiac, cochlea, and neural tube development in mice. DVL3, like other members of the DVL family, functions upstream of glycogen synthase kinase-3 (GSK3) to modulate the canonical wnt pathway. A frame shift mutation in DVL3 gene is implicated in Robinow syndrome. Allelic variation in the DVL3 gene may be associated with major depressive disorder.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The dopamine D2 receptor regulates Akt and GSK-3 via Dvl-3
Sutton LP and Rushlow WJ
The International Journal of Neuropsychopharmacology, 15(7), 965-979 (2012)
Integrating expression-related SNPs into genome-wide gene-and pathway-based analyses identified novel lung cancer susceptibility genes
Wang Y, et al.
International journal of cancer management, 142(8), 1602-1610 (2018)
DVL3 alleles resulting in a- 1 frameshift of the last exon mediate autosomal-dominant Robinow syndrome
White JJ, et al.
American Journal of Human Genetics, 98(3), 553-561 (2016)
Murine dishevelled 3 functions in redundant pathways with dishevelled 1 and 2 in normal cardiac outflow tract, cochlea, and neural tube development
Etheridge SL, et al.
PLoS Genetics, 4(11), e1000259-e1000259 (2008)
Comparative phosphorylation map of Dishevelled 3 links phospho-signatures to biological outputs
Hanakova K, et al.
Cell communication and signaling : CCS, 17(1), 170-170 (2019)

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