P9290
Proteinase K–Agarose from Tritirachium album
lyophilized powder
Synonym(s):
Proteinase K-Agarose
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10 UNITS
$417.00
25 UNITS
$909.00
$417.00
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About This Item
Form:
lyophilized powder
Biological source:
microbial (T.album
T. ALBUM)
T. ALBUM)
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biological source
microbial (T.album
T. ALBUM)
Quality Level
form
lyophilized powder
extent of labeling
30-140 units per g solid
technique(s)
DNA extraction: suitable, RNA extraction: suitable, protein extraction: suitable
swelling
1 g swells to ~5 mL
storage temp.
−20°C
1 of 4
This Item | |||
|---|---|---|---|
| technique(s) DNA extraction: suitable, protein extraction: suitable, RNA extraction: suitable | technique(s) - | technique(s) - | technique(s) DNA extraction: suitable |
| biological source microbial (T.album | biological source Staphylococcus aureus | biological source - | biological source - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 400 | Quality Level 300 |
| extent of labeling 30-140 units per g solid | extent of labeling ~2 mg per mL | extent of labeling - | extent of labeling - |
Application
Proteinase K-agarose is used in protein and nucleic acid isolation, modifying enzymes and immobilized enzymes. Proteinase K-agarose has been used to study Klebsiella pneumonia as well as Echinococcus multilocularis infection in domesticated dogs.
Biochem/physiol Actions
The application of proteinase K has been extended by immobilizing it on an insoluble matrix such as agarose. The agarose is quite inert in most systems and allows high activities per unit weight. This reactive resin allows the enzyme reaction to be quickly catalyzed by momentary contact with the substrate in a suitable medium. Reactions can be carried out in a batch slurry or even through a small column. In either case, the insoluble enzyme does not contaminate the reaction mix, which can be readily filtered out of the batch slurry or remain behind in the column.
Features and Benefits
• Recylable Proteinase K on matrix support.
• Spin column or batch processing possible.
• Spin column or batch processing possible.
Physical form
Lyophilized powder stabilized with lactose
Other Notes
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Yi Zuo et al.
PloS one, 14(10), e0223299-e0223299 (2019-10-04)
Oral herpes is a highly prevalent infection caused by herpes simplex virus 1 (HSV-1). After an initial infection of the oral cavity, HSV-1 remains latent in sensory neurons of the trigeminal ganglia. Episodic reactivation of the virus leads to the
Hirokazu Kouguchi et al.
Experimental parasitology, 128(1), 50-56 (2011-02-09)
We show that a conventionally purified glycoprotein component of Echinococcus multilocularis protoscolex, designated as Emgp-89, may be useful as a serodiagnostic antigen for detecting E. multilocularis infection in dogs domesticated in endemic areas. Emgp-89 was obtained from the parasite material
P Champeil et al.
The Journal of biological chemistry, 273(12), 6619-6631 (1998-04-18)
Treatment of rabbit sarcoplasmic reticulum Ca2+-ATPase with a variety of proteases, including elastase, proteinase K, and endoproteinases Asp-N and Glu-C, results in accumulation of soluble fragments starting close to the ATPase phosphorylation site Asp351 and ending in the Lys605-Arg615 region
David da Silva Barreira et al.
Scientific reports, 13(1), 1163-1163 (2023-01-21)
Biofilms represent a major concern in the food industry and healthcare. The use of probiotic bacteria and their derivatives as an alternative to conventional treatments to fight biofilm development is a promising option that has provided convincing results in the
Shai Eyal et al.
Development (Cambridge, England), 146(4) (2019-02-13)
Sesamoid bones are small auxiliary bones that form near joints and contribute to their stability and function. Thus far, providing a comprehensive developmental model or classification system for this highly diverse group of bones has been challenging. Here, we compare
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| P9290-10UN | 04061824824414 |
| P9290-25UN | 04061824824421 |
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