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P0498

Anti-PRL-3 antibody produced in rabbit

Name: Anti-PRL-3 antibody produced in rabbit
Brand: SIGMA

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-PTP4A3, Anti-Phosphatase of Regenerating Liver

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About This Item

MDL number:

MFCD07370459

UNSPSC Code:

12352203

NACRES:

NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~25 kDa

species reactivity

human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:250-1:500 using protease digested tissue sections from human heart by biotin/ExtrAvidin^®-peroxidase staining
immunoprecipitation (IP): 0.5-1 μL using preparation containing recombinant human PRL-3
western blot (chemiluminescent): 1:500-1:1,000 using whole extract of human HepG2 cells

UniProt accession no.

O75365

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PTP4A3(11156)
mouse ... Ptp4a3(19245)

Related Categories

Primary Antibodies

General description

Phosphatase of regenerating liver (PRL-3), also known as protein tyrosine phosphatase type IVA, member 3 (PTP4A3) is a dual-specificity phosphatase. It belongs to the phosphatases of regenerating liver family. The PTP4A3 gene is localized on human chromosome 8q24.3.

Specificity

Anti-PRL-3 recognizes human phosphatase of regenerating live (PRL)-3 and does not cross-react with human PRL-1 and PRL-2.

Immunogen

Synthetic peptide corresponding to amino acid residues of human PRL-3 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse.

Application

Anti-PRL-3 antibody produced in rabbit has been used in western blotting and immunohistochemistry.

Biochem/physiol Actions

Phosphatase of regenerating liver (PRL-3) promotes epithelial-mesenchymal transition. It also plays an essential role in cancer cell migration, invasion and metastasis. It dephosphorylates serine, threonine and tyrosine residues. The protein is overexpressed in various cancers. PTP4A3 associates with transcription factors and regulates gene transcription. In addition, it also regulates breast cancer cell proliferation and invasion. Thus, this protein acts as a potent therapeutic target for breast cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Expression of PRL-3 regulates proliferation and invasion of breast cancer cells in vitro.

Radke I, et al.

Archives of Gynecology and Obstetrics (2017)

Pax3 and Zic1 trigger the early neural crest gene regulatory network by the direct activation of multiple key neural crest specifiers.

Jean-Louis Plouhinec et al.

Developmental biology, 386(2), 461-472 (2013-12-24)

Neural crest development is orchestrated by a complex and still poorly understood gene regulatory network. Premigratory neural crest is induced at the lateral border of the neural plate by the combined action of signaling molecules and transcription factors such as

Expression of phosphatase of regenerating liver (PRL)-3, is independently associated with biochemical failure, clinical failure and death in prostate cancer.

Andersen S, et al.

PLoS ONE, 12(11), e0189000-e0189000 (2017)

High PTP4A3 Phosphatase Expression Correlates with Metastatic Risk in Uveal Melanoma Patients

Laurent C, et al.

Cancer Research, 71(3), 666-674 (2011)

Src-Mediated Phosphorylation of the Tyrosine Phosphatase PRL-3 Is Required for PRL-3 Promotion of Rho Activation, Motility and Invasion

Fiordalisi JJ, et al.

PLoS ONE, 8(5), e64309-e64309 (2013)

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