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MOPS-EDTA-Sodium Acetate (MESA)

Name: MOPS-EDTA-Sodium Acetate (MESA)
Brand: SIGMA

powder for RNA electrophoresis buffers

Synonym(s):

MESA Buffer

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About This Item

NACRES:

NA.25

UNSPSC Code:

12161700

Pricing and availability is not currently available.

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grade

Molecular Biology

Quality Level

200

product line

BioReagent

form

powder

technique(s)

electrophoresis: suitable

pH

6.8-7.2(reconstituted per directions)

solubility

H₂O: soluble, faintly yellow

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

room temp

Related Categories

Biochemicals

Biological Buffers

Nucleic Acid Electrophoresis & Hybridization

Nucleic Acid Gel Electrophoresis Equipment and Systems

Chemistry & Biochemicals

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Show Differences

1 of 1

This Item	T8280	T7527	PPB008
Sigma-Aldrich M5755 MOPS-EDTA-Sodium Acetate (MESA)	Sigma-Aldrich T8280 Tris Acetate-EDTA buffer	Sigma-Aldrich T7527 Tris-Borate-EDTA buffer	Sigma-Aldrich PPB008 Tris Acetate-EDTA buffer
grade Molecular Biology	grade Molecular Biology	grade Molecular Biology	grade -
technique(s) electrophoresis: suitable	technique(s) -	technique(s) -	technique(s) -
product line BioReagent	product line BioReagent	product line BioReagent	product line pHast Pack^™
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level -
form powder	form powder blend	form powder blend	form powder
pH 6.8-7.2(reconstituted per directions)	pH 8.1-8.5	pH 8.1-8.5 (5 ×)	pH 8.3

General description

MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.

Application

Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.

Preparation Note

1x MESA buffer contains:

40 mM MOPS
10 mM sodium acetate
1 mM EDTA (pH 8.3)

Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000532868

0000439909

0000383887

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Newly identified cytochrome c oxidase operon in the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 specifically induced in heterocysts.

Kathryn M Jones et al.

Journal of bacteriology, 184(9), 2491-2499 (2002-04-12)

Two operons have been cloned from Anabaena sp. strain PCC 7120 DNA, each of which encodes the three core subunits of distinct mitochondrial-type cytochrome c oxidases. The two operons are only 72 to 85% similar to one another at the

Sambrook, J., et al.

Molecular Cloning: A Laboratory Manual, 7-7 (1989)

Molecular techniques for detection of gene expression in neuroimmunology.

Cowan, E.P., and Dhib-Jalbut, S.S.

Methods in Neuroscience, 24, 41-41 (1995)

The sperm chemoattractant "allurin" is expressed and secreted from the Xenopus oviduct in a hormone-regulated manner.

Xueyu Xiang et al.

Developmental biology, 275(2), 343-355 (2004-10-27)

Recently, we cloned and sequenced the cDNA of allurin, a sperm chemoattractant isolated from the jelly of Xenopus laevis eggs [Proc. Natl. Acad. Sci. U.S.A. 78 (2001) 11205]. In this report, we demonstrate that allurin mRNA is expressed almost exclusively

Electrophoresis in agarose and acrylamide gels.

R C Ogden et al.

Methods in enzymology, 152, 61-87 (1987-01-01)

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Global Trade Item Number

SKU	GTIN
M5755-1L	04061834060314

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