L8918

Anti-MAP1LC3A antibody

Name: Anti-MAP1LC3A antibody
Brand: SIGMA

rabbit polyclonal

Synonym(s):

Anti-Lc3, LC3 Antibody - Anti-LC3 antibody produced in rabbit, Lc3 Antibody, Anti-MAP1 light chain 3-like protein 1, Anti-MAP1A/1B light chain 3A, Anti-MAP1A/1BLC3, Anti-MAP1A/MAP1B LC3A, Anti-MAP1ALC3, Anti-MAP1BLC3, Anti-MAP1LC3A, Anti-MAP1LC3B, Anti-Microtubule-associated protein 1 light chain 3 alpha, Anti-Microtubule-associated protein 1 light chain 3 beta, Anti-Microtubule-associated proteins 1A/1B light chain 3

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IHC, IP, WB

Citations:

345

Key Documents

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Product Name

Anti-LC3 antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 16-18 kDa

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): 5-10 μg using extracts of human U87 cells, western blot: 1-2 μg/mL using whole extracts of rat and mouse brain

UniProt accession no.

Q9H492

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAP1LC3A(84557), MAP1LC3B(81631)
mouse ... Map1lc3a(66734), Map1lc3b(67443)
rat ... Map1lc3a(362245), Map1lc3b(64862)

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Show Differences

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This Item	L7543	SAB4200361	SAB1305638
Sigma-Aldrich L8918 Anti-LC3 antibody produced in rabbit	L7543 Anti-LC3B antibody produced in rabbit	Sigma-Aldrich SAB4200361 Anti-LC3B antibody, Mouse monoclonal	$ANTI-LC3 (APG8A) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich SAB1305638 ANTI-LC3 (APG8A) antibody produced in rabbit
biological source rabbit	biological source rabbit	biological source mouse	biological source rabbit
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form IgG fraction of antiserum
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate -
UniProt accession no. Q9H492	UniProt accession no. Q9GZQ8	UniProt accession no. Q9GZQ8	UniProt accession no. Q9H492
mol wt antigen 16-18 kDa	mol wt antigen 16-18 kDa	mol wt antigen ~18/16 kDa	mol wt 14272 Da

General description

The gene LC3, also referred to as MAP1LC3A (microtubule associated protein 1 light chain 3 α), is a homologue of Apg8p necessary for autophagy in yeast. The cytosolic form upon processing gets localized to the autophagosome membranes. The gene is mapped to human chromosome 20q11.22. LC3 exists in cells as cytoplasmic LC3-I (18kDa) and LC3-II (16kDa).

Immunogen

Synthetic peptide corresponding to amino acids of human LC3A isoform a, conjugated to KLH via C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Application

Anti-LC3 antibody produced in rabbit has been used in:

western blotting[1][2][3]
immunofluorescence[4]
immunohistochemistry[5]
filter retardation assay
immunocytochemistry[6]

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (3 papers)
Immunohistochemistry (1 paper)
Western Blotting (21 paper)

Biochem/physiol Actions

The gene MAP1LC3A (microtubule associated protein 1 light chain 3 α) encodes a protein that serves as a marker of autophagosomes. It is found to be expressed on surviving oligodendrocytes in Nasu-Hakola disease brains and may be involved in the induction of oligodendrogliopathy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000527518

0000488229

0000492451

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TRIAD1 Is a Novel Transcriptional Target of p53 and Regulates Nutlin-3a-Induced Cell Death.

Lee J

Journal of Cellular Biochemistry (2016)

Lysosomal abnormalities in hereditary spastic paraplegia types SPG15 and SPG11.

Benoît Renvoisé et al.

Annals of clinical and translational neurology, 1(6), 379-389 (2014-07-08)

Hereditary spastic paraplegias (HSPs) are among the most genetically diverse inherited neurological disorders, with over 70 disease loci identified (SPG1-71) to date. SPG15 and SPG11 are clinically similar, autosomal recessive disorders characterized by progressive spastic paraplegia along with thin corpus

LC3, an autophagosome marker, is expressed on oligodendrocytes in Nasu-Hakola disease brains.

Satoh J

Orphanet Journal of Rare Diseases, 9 (2014)

Endoplasmic Reticulum Stress in Heat- and Shake-Induced Injury in the Rat Small Intestine.

Peng Yin et al.

PloS one, 10(12), e0143922-e0143922 (2015-12-05)

We investigated the mechanisms underlying damage to rat small intestine in heat- and shake-induced stress. Eighteen Sprague-Dawley rats were randomly divided into a control group and a 3-day stressed group treated 2 h daily for 3 days on a rotary

Dual effects of gossypol on human hepatocellular carcinoma via endoplasmic reticulum stress and autophagy.

Guang Zhang et al.

The international journal of biochemistry & cell biology, 113, 48-57 (2019-05-28)

Treatment outcomes for hepatocellular carcinoma (HCC) remain unsatisfactory, and effective new therapeutic methods are urgently needed. Gossypol has been shown to have an anti-HCC effect, but the underlying mechanism requires further study. In this study, we found gossypol inhibited HCC

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Global Trade Item Number

SKU	GTIN
L8918-200UL	04061833966303

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