KCQS01
KiCqStart® SYBR® Green qPCR ReadyMix™
Low ROX™, for ABI and Stratagene instruments
Synonym(s):
qPCR master mix, sybr green qPCR
Select a Size
| Pack Size | SKU | Availability | Price |
|---|
About This Item
form
liquid
usage
sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions
feature
dNTPs included, hotstart
storage condition
protect from light
technique(s)
RT-qPCR: suitable, qPCR: suitable
color
colorless
input
purified DNA
crude DNA
compatibility
for use with ABI 7500 Fast, for use with ABI 7500, for use with ABI ViiA 7, for use with Agilent AriaMx, for use with Douglas Scientific IntelliQube, for use with Qiagen Rotor-Gene Q, for use with QuantStudio™, for use with Strategene Mx3000P, for use with Strategene Mx3005P, for use with Strategene Mx4000
detection method
SYBR® Green
shipped in
dry ice
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This Item | |||
|---|---|---|---|
| technique(s) RT-qPCR: suitable | technique(s) RT-qPCR: suitable | technique(s) RT-qPCR: suitable | technique(s) RT-qPCR: suitable |
| compatibility for use with ABI 7500 Fast, for use with ABI ViiA 7, for use with Douglas Scientific IntelliQube, for use with QuantStudio™, for use with Strategene Mx3005P, for use with Qiagen Rotor-Gene Q, for use with Strategene Mx4000, for use with Agilent AriaMx, for use with Strategene Mx3000P | compatibility for use with Bio-Rad CFX384, for use with Bio-Rad CFX96, for use with Bio-Rad MJ Chromo4, for use with Bio-Rad MJ Opticon 2, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Bio-Rad MiniOpticon, for use with Bio-Rad MyiQ, for use with Eppendorf® Mastercycler ep realplex2 s, for use with Illumina Eco qPCR, for use with Qiagen Corbett Rotor-Gene 3000, for use with Qiagen Corbett Rotor-Gene 6000, for use with Qiagen Corbett Rotor-Gene Q, for use with Roche LightCycler 480 | compatibility for use with ABI 5700, for use with ABI 7000, for use with ABI 7300, for use with ABI 7700, for use with ABI 7900 HT Fast, for use with ABI StepOne, for use with ABI StepOnePlus | compatibility for use with Bio-Rad MyiQ, for use with Bio-Rad iCycler iQ, for use with Bio-Rad iQ 5 |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| form liquid | form liquid | form liquid | form liquid |
| input purified DNA | input purified DNA | input purified DNA | input purified DNA |
| feature dNTPs included, hotstart | feature dNTPs included, hotstart | feature dNTPs included, hotstart | feature dNTPs included, hotstart |
General description
Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.
Application
- for the amplification and quantification of DNA in real-time PCR (qPCR) assay[1]
- in the amplification and quantification of transcripts in 2-step quantitative reverse transcription polymerase chain reaction (qRT-PCR)[2]
- in the amplification of complementary DNA (cDNA) by real-time PCR (qPCR) assay[3]
- Gene expression
- DNA quantification
- CHiP
Features and Benefits
- Assay results in as little as 33 minutes
- Highly efficient and sensitive real-time PCR results
- Little/no optimization required
Analysis Note
Other Notes
packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.
Legal Information
related product
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
Watch these videos to learn how real time or quantitative PCR (qPCR) works and the benefits of both the SYBR Green-based and probe-based methods of qPCR assay.
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
Protocols
Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.
SPUD assay identifies inhibitors in RNA or DNA samples, useful for analyzing numerous or low-copy targets.
A protocol that can be used as a basic template for qPCR incorporating SYBR Green I DNA binding dye that is amenable to modification and applicable for use as validation for a set of primers.
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| KCQS01-SAMPLE | 04061838099471 |
| KCQS01-5000RXN | 04061835573318 |
| KCQS01-250RXN | 04061835512669 |
| KCQS01-1250RXN | 04061835573301 |
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