KCQS00
KiCqStart® SYBR® Green qPCR ReadyMix™
For Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
Synonym(s):
qPCR master mix, sybr green qPCR
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 250 reactions | Check Cart for Availability | $369.00 | |
| 1250 reactions | Check Cart for Availability | $1,650.00 |
About This Item
$369.00
form
liquid
usage
sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions
feature
dNTPs included, hotstart
storage condition
protect from light
technique(s)
RT-qPCR: suitable, qPCR: suitable
color
colorless
input
purified DNA
compatibility
for use with Bio-Rad CFX384, for use with Bio-Rad CFX96, for use with Bio-Rad MJ Chromo4, for use with Bio-Rad MJ Opticon 2, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Bio-Rad MiniOpticon, for use with Bio-Rad MyiQ, for use with Eppendorf® Mastercycler ep realplex2 s, for use with Eppendorf® Mastercycler ep realplex, for use with Illumina Eco qPCR, for use with Qiagen Corbett Rotor-Gene 3000, for use with Qiagen Corbett Rotor-Gene 6000, for use with Qiagen Corbett Rotor-Gene Q, for use with Roche LightCycler 480
detection method
SYBR® Green
shipped in
dry ice
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1 of 1
This Item | |||
|---|---|---|---|
| technique(s) RT-qPCR: suitable | technique(s) RT-qPCR: suitable | technique(s) RT-qPCR: suitable | technique(s) RT-qPCR: suitable |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| color colorless | color colorless | color colorless | color light blue |
| input purified DNA | input purified DNA | input purified DNA | input purified RNA |
| compatibility for use with Bio-Rad CFX384, for use with Bio-Rad MJ Chromo4, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Bio-Rad MyiQ, for use with Eppendorf® Mastercycler ep realplex, for use with Qiagen Corbett Rotor-Gene 3000, for use with Qiagen Corbett Rotor-Gene Q, for use with Bio-Rad CFX96, for use with Bio-Rad MiniOpticon, for use with Illumina Eco qPCR, for use with Roche LightCycler 480, for use with Bio-Rad MJ Opticon 2, for use with Qiagen Corbett Rotor-Gene 6000, for use with Eppendorf® Mastercycler ep realplex2 s | compatibility for use with ABI 7500 Fast, for use with ABI ViiA 7, for use with Agilent AriaMx, for use with Douglas Scientific IntelliQube, for use with Qiagen Rotor-Gene Q, for use with QuantStudio™, for use with Strategene Mx3000P, for use with Strategene Mx3005P, for use with Strategene Mx4000 | compatibility for use with ABI 5700, for use with ABI 7000, for use with ABI 7300, for use with ABI 7700, for use with ABI 7900 HT Fast, for use with ABI StepOne, for use with ABI StepOnePlus | compatibility Bio-Rad CFX384, Bio-Rad CFX96, Bio-Rad MJ Chromo4, Bio-Rad MJ Opticon 2, Bio-Rad MiniOpticon, Eppendorf® Mastercycler ep realplex2 s, Illumina Eco qPCR, Qiagen Corbett Rotor-Gene 3000, Qiagen Corbett Rotor-Gene 6000, Qiagen Corbett Rotor-Gene Q, Roche LightCycler 480 |
| usage sufficient for 1250 reactions, sufficient for 5000 reactions, sufficient for 250 reactions | usage sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions | usage sufficient for 1250 reactions, sufficient for 250 reactions, sufficient for 5000 reactions | usage sufficient for 500 reactions |
General description
Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.
Application
- Gene expression
- DNA quantification
- CHiP
Features and Benefits
- Assay results in as little as 33 minutes
- Highly efficient and sensitive real-time PCR results
- Little/no optimization required
Other Notes
packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:
- Bio-Rad® CFX384™
- Bio-Rad CFX96™
- Bio-Rad MiniOpticon™
- Bio-Rad/MJ Chromo4™
- Bio-Rad/MJ Opticon 2
- Bio-Rad/MJ Opticon™
- Cepheid SmartCycler®
- Eppendorf Mastercycler® ep realplex
- Eppendorf Mastercycler ep realplex2 s
- Illumina Eco qPCR
- Qiagen/Corbett Rotor-Gene® 3000
- Qiagen/Corbett Rotor-Gene 6000
- Qiagen/Corbett Rotor-Gene Q
- Roche LightCycler® 480
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.
Legal Information
related product
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR
PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
Protocols
Quantitative PCR protocol using SYBR Green reagents. Procedure supports most qPCR instruments.
Gradient PCR optimizes assay conditions by testing fixed primer concentrations across various annealing temperatures.
Related Content
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR
Global Trade Item Number
| SKU | GTIN |
|---|---|
| KCQS00-1250RXN | 04061833945087 |
| KCQS00-5000RXN | 04061833253502 |
| KCQS00-250RXN | 04061833945094 |
| KCQS00-SAMPLE | 04061838099464 |
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