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β-Glucuronidase from limpets (Patella vulgata)

Name: β-Glucuronidase from limpets (<I>Patella vulgata</I>)
Brand: SIGMA
Price: 85.5 USD

Type L-II, lyophilized powder, 1,000,000-3,000,000 units/g solid

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

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Pack Size	Availability	Price
100000 units	Check Cart for Availability	$85.50
250000 units	Check Cart for Availability	$170.00
500000 units	Check Cart for Availability	$284.00
1000000 units	Check Cart for Availability	$365.00
2000000 units	Check Cart for Availability	$582.00
5 x 1 ea	Check Cart for Availability	$1,300.00

About This Item

CAS Number:

9001-45-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-606-8

MDL number:

MFCD00130629

EC Number:

3.2.1.31 (BRENDA, IUBMB)

Specific activity:

1,000,000-3,000,000 units/g solid

Biological source:

limpet (Patella vulgata)

$85.50

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biological source

limpet (Patella vulgata)

Quality Level

300

type

Type L-II

form

lyophilized powder

specific activity

1,000,000-3,000,000 units/g solid

shipped in

wet ice

storage temp.

−20°C

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Related Categories

Drug Analysis Enzymes

Protein Biology

Proteins & Enzymes

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Show Differences

1 of 1

This Item	G0751	G0251	G7396
Sigma-Aldrich G8132 β-Glucuronidase from limpets (Patella vulgata)	Sigma-Aldrich G0751 β-Glucuronidase from Helix pomatia	Sigma-Aldrich G0251 β-Glucuronidase from bovine liver	Sigma-Aldrich G7396 β-Glucuronidase from Escherichia coli
biological source limpet (Patella vulgata)	biological source -	biological source -	biological source -
specific activity 1,000,000-3,000,000 units/g solid	specific activity ≥300,000 units/g solid	specific activity ≥1,000,000 units/g solid	specific activity 1,000,000-5,000,000 units/g protein (30 min assay)
form lyophilized powder	form partially purified powder	form solid	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
shipped in wet ice	shipped in -	shipped in -	shipped in wet ice
Quality Level 300	Quality Level 200	Quality Level 200	Quality Level 300

Application

β-Glucuronidase Type L-II has been shown to be a superior enzyme for the hydrolysis of drug-glucuronides from urine. [1]

New Technical Article Comparing Performance of Different Enzymes
Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes

Biochem/physiol Actions

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates.[2] In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption. Morphine 3-β-D glucuronide was converted to morphine using β-glucuronidase obtained from Patella vulgate.

Analysis Note

Contains sulfatase activity which is inhibited by 0.1 M phosphate.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

used together

Product No.

Description

Pricing

P0501

Phenolphthalein β-D-glucuronide, β-glucuronidase substrate

B8174

5-Bromo-4-chloro-3-indolyl β-D-glucuronide sodium salt, tablet

B4532

5-Bromo-6-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, ≥98%

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Comparison of clonazepam compliance by measurement of urinary concentration by immunoassay and LC-MS/MS in pain management population.

Robert West et al.

Pain physician, 13(1), 71-78 (2010-02-02)

Physicians determine patient compliance with their medications by use of urine drug testing. It is known that measurement of benzodiazepines is limited by immunoassay specificity and cutoff limits and therefore does not offer physicians an accurate picture of their patients'

Vectors with the gus reporter gene for identifying and quantitating promoter regions in <I>Saccharomyces cerevisiae</I>

S.V. Marathe & J.E. McEwen

Gene, 154, 105-107 (1994)

Mechanisms of enzymatic glycoside hydrolysis.

J D McCarter et al.

Current opinion in structural biology, 4(6), 885-892 (1994-12-01)

The determination of a large number of three-dimensional structures of glycosidases, both free and in complex with ligands, has provided valuable new insights into glycosidase catalysis, especially when coupled with results from studies of specifically labelled glycosidases and kinetic analyses

Coupling Complete Blood Count and Steroidomics to Track Low Doses Administration of Recombinant Growth Hormone: An Anti-Doping Perspective.

Luca Narduzzi et al.

Frontiers in molecular biosciences, 8, 683675-683675 (2021-06-29)

Growth Hormone (GH) under its human recombinant homologue (rhGH), may be abused by athletes to take advantage of its well-known anabolic and lipolytic properties; hence it is prohibited in sports by the World Anti-Doping Agency. Due to the rapid turnover

Morphine glucuronide hydrolysis: superiority of beta-glucuronidase from Patella vulgata.

J Combie et al.

Clinical chemistry, 28(1), 83-86 (1982-01-01)

beta-Glucuronidase from Patella vulgata, Helix aspersa, Helix pomatia, and bovine liver were evaluated for usefulness in routine hydrolysis of drug-glucuronic acid conjugates from equine urine samples. Factors affecting the reaction rate (enzyme concentration, ligand concentration, temperature, and pH) were optimized.

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Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Analysis of Drugs of Abuse in Urine

Validate urine sample cleanup with HLB SPE for opioid analysis via LC-MS/MS using the Supel^™ Swift HLB 96-well plate.

Protocols

ß-Glucuronidase Enzymatic Assay

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

UHPLC/MS for Drug Detection in Urine

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

Global Trade Item Number

SKU	GTIN
G8132-2MU	04061833643679
G8132-500KU	04061833643686
G8132-250KU	04061833643662
G8132-100KU	04061835557059
G8132-1MU	04061835557066
G8132-5X1MU	04061833643693

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