Skip to Content
MilliporeSigma
Get 22% off for Pi Day through March 31.Save Now

Key Documents

View All Documentation

Skip To

G2174

β-Glucuronidase from limpets (Patella vulgata)

aqueous solution, ≥85,000 units/mL

Synonym(s):

β-D-Glucuronide glucuronosohydrolase

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice
1 mL
Check Cart for Availability
$80.80
5 mL
Check Cart for Availability
$269.00
10 mL
Check Cart for Availability
$456.00
25 mL
Check Cart for Availability
$910.00

About This Item

CAS Number:
9001-45-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.2.1.31 (BRENDA, IUBMB)
MDL number:
MFCD00130629
Specific activity:
≥85,000 units/mL
Biological source:
limpet (Patella vulgata)

$80.80

Check Cart for Availability
​
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Level

200

biological source

limpet (Patella vulgata)

form

aqueous solution

specific activity

≥85,000 units/mL

secondary activity

≤7,500 units/mL sulfatase

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
SRE0093G8132G7770
β-Glucuronidase from limpets (Patella vulgata) aqueous solution, ≥85,000 units/mL

Sigma-Aldrich

G2174

β-Glucuronidase from limpets (Patella vulgata)

β-Glucuronidase from limpets (Patella vulgata) aqueous solution

Sigma-Aldrich

SRE0093

β-Glucuronidase from limpets (Patella vulgata)

β-Glucuronidase from limpets (Patella vulgata) Type L-II, lyophilized powder, 1,000,000-3,000,000 units/g solid

Sigma-Aldrich

G8132

β-Glucuronidase from limpets (Patella vulgata)

β-Glucuronidase from Helix pomatia Type HP-2S, aqueous solution, ≥90,000 units/mL

Sigma-Aldrich

G7770

β-Glucuronidase from Helix pomatia

specific activity

≥85,000 units/mL

specific activity

-

specific activity

1,000,000-3,000,000 units/g solid

specific activity

≥90,000 units/mL

biological source

limpet (Patella vulgata)

biological source

-

biological source

limpet (Patella vulgata)

biological source

-

form

aqueous solution

form

aqueous solution

form

lyophilized powder

form

aqueous solution

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

200

secondary activity

≤7,500 units/mL sulfatase

secondary activity

-

secondary activity

-

secondary activity

≤7,500 units/mL sulfatase

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes

Features and Benefits

Save time with no reconstitution in this convenient liquid format.

Physical form

Aqueous solution in 0.9% NaCl with 0.02% sodium azide as preservative.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000545841
0000532499
0000545841
0000532499
0000512111

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Vanessa Andrea Meling et al.
Molecules (Basel, Switzerland), 27(8) (2022-04-24)
The aquaculture industry has become a sustainable source of food for humans. Remaining challenges include disease issues and ethical concerns for the discomfort and stress of farmed fish. There is a need for reliable biomarkers to monitor welfare in fish
J Combie et al.
Clinical chemistry, 28(1), 83-86 (1982-01-01)
beta-Glucuronidase from Patella vulgata, Helix aspersa, Helix pomatia, and bovine liver were evaluated for usefulness in routine hydrolysis of drug-glucuronic acid conjugates from equine urine samples. Factors affecting the reaction rate (enzyme concentration, ligand concentration, temperature, and pH) were optimized.
T A Jennison et al.
Journal of analytical toxicology, 17(4), 208-210 (1993-07-01)
Methods to confirm morphine in urine require hydrolysis to liberate morphine from its 3-beta-D glucuronide (M-3G) conjugate. Lengthy enzyme hydrolysis procedures prolong testing turnaround time whereas rapid enzyme methods may produce a low conversion of M-3G to morphine. The purpose
Victor Aguilar-Hernández et al.
Plant molecular biology, 84(4-5), 429-441 (2013-10-19)
Gene duplication events exert key functions on gene innovations during the evolution of the eukaryotic genomes. A large portion of the total gene content in plants arose from tandem duplications events, which often result in paralog genes with high sequence
Miribane Dërmaku-Sopjani et al.
Molecular membrane biology, 30(8), 369-385 (2013-10-16)
The Klotho gene was identified as an 'aging suppressor' in mice. Overexpression of the Klotho gene extends lifespan and defective Klotho results in rapid aging and early death. Both the membrane and secreted forms of Klotho have biological activity that
View All Related Papers

Articles

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Protocols

UHPLC/MS for Drug Detection in Urine

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

LC/MS (TOF) Analysis of Drugs and Their Glucuronide Metabolites in Urine on Titan™ C18 After Solid Phase Extraction (SPE) Using Supel™-Select SCX, β-Glucuronidase Enzyme Digestion

-THC solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material

Related Content

Data Sheet - G2174

Instructions

Global Trade Item Number

SKUGTIN
G2174-1ML04061838087447
G2174-5ML04061838087454
G2174-25ML04061833628737
G2174-10ML04061838087430

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service