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Anti-Atg14 antibody produced in rabbit

Name: Anti-Atg14 antibody produced in rabbit
Brand: SIGMA

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-KIAA0831

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

polyclonal

Application:

Citations:

This product is discontinued. Contact Technical Servicefor assistance.

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~60 kDa

species reactivity

mouse, human

technique(s)

western blot: 1:150-1:300 using whole extracts of mouse 3T3 cells, western blot: 1:250-1:500 using whole extracts of human HeLa cells.

shipped in

dry ice

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This Item	A7481	R5530	I0505
$Anti-Atg14 antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich A6358 Anti-Atg14 antibody produced in rabbit	Sigma-Aldrich A7481 Anti-Atg1/ULK1 antibody produced in rabbit	$Anti-Rab8 antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich R5530 Anti-Rab8 antibody produced in rabbit	$Anti-IκBα antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich I0505 Anti-IκBα antibody produced in rabbit
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form IgG fraction of antiserum
mol wt antigen ~60 kDa	mol wt antigen ~150 kDa	mol wt antigen ~24 kDa	mol wt antigen 36 kDa
Gene Information human ... KIAA0831(22863)	Gene Information human ... ULK1(8408) mouse ... Ulk1(22241) rat ... Ulk1(360827)	Gene Information human ... RAB8A(4218) mouse ... Rab8a(17274) rat ... Rab8a(117103)	Gene Information human ... NFKBIA(4792) mouse ... Nfkbia(18035) rat ... Nfkbia(25493)
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

General description

Autophagy-specific subunit of the PtdIns3K complex (Atg14) is encoded by the gene mapped to human chromosome 14q22.3. Atg14, also known as KIAA0831, is an autophagy protein.

Application

Anti-Atg14 antibody produced in rabbit has been used in western blotting.[1]

Biochem/physiol Actions

Anti-Atg14 recognizes human and mouse Atg14.

Autophagy Related 14 (Atg14) is an essential gene for autophagy. Atg14 is present on autophagic isolation membranes and is required for autophagosome formation. Atg14 together with vacuolar protein sorting-associated protein 34 (Vps34) and Atg6/Vps30 forms part of a phosphoinositide 3(PI3)-kinase complex in yeast. Two distinct PI3-kinase complexes, complex I and II, were identified. Complex I is involved in autophagy, whereas complex II is involved in vacuolar protein sorting. In mammals, homologs of Vps34, Vps15, Vps30/Atg6, Atg14 and Vps38 were identified as Vps34, p150, Beclin 1, Atg14 and UV radiation resistance associated gene (UVRAG), respectively. Although both Atg14 and UVRAG interact with Beclin 1, they are not present in the same complex. While Atg14 is present on autophagic isolation membranes, UVRAG mainly localized to Ras-related protein Rab9-positive endocytic compartments.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number

105M4829V

113M4829

118K4767

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Investigation of Androgen Responsive Elements in Some Autophagy Related Genes via In Silico Analysis.

Erzurumlu Y

S Int. Rev. Immunol., 11(1) (2020)

Angiotensin II suppresses autophagy and disrupts ultrastructural morphology and function of mitochondria in mouse skeletal muscle

KAS Silva, et al.

Journal of Applied Physiology, 126(6), 1550-1562 (2019)

Atg14 and UVRAG: mutually exclusive subunits of mammalian Beclin 1-PI3K complexes

Itakura E and Mizushima N

Autophagy, 5(4), 534-536 (2009)

Critical role of FoxO3a in alcohol-induced autophagy and hepatotoxicity.

Hong-Min Ni et al.

The American journal of pathology, 183(6), 1815-1825 (2013-10-08)

Autophagy is a lysosomal degradation process that degrades long-lived cellular proteins and damaged organelles as a critical cell survival mechanism in response to stress. We recently reported that acute ethanol induces autophagy, which then reduces ethanol-induced liver injury. However, the

Atg14: a key player in orchestrating autophagy

Obara K and Ohsumi Y

International Journal of Cell Biology, 2011 (2011)

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Global Trade Item Number

SKU	GTIN
A6358-200UL	04061838029683

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