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A4227

Sigma-Aldrich

Anti-ASAP1/Centaurin β4 (ED-20) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-Arf GAP containing SH3, ANK repeat, and PH domains/Centaurin β4 (ED-20), DEF1

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About This Item

MDL number:
MFCD04040184
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 130 kDa (doublet band)

species reactivity

rat, mouse, human

technique(s)

immunofluorescence: 1:1,000 using mouse NIH3T3 fibroblast cells
microarray: suitable
western blot: 1:2,500 using rat brain and mouse brain extracts

UniProt accession no.

Q9ULH1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ASAP1(50807)
mouse ... Asap1(13196)
rat ... Asap1(314961)

General description

ASAP1 is a phospholipid-dependent ARF GTPase-activating protein that activates ARF for membrane trafficking regulation.
Rabbit anti-ASAP1/Centaurin β4 (ED-20) antibody recognizes human ASAP1 by immunoblotting (130kDa, doublet band). Staining of ASAP1 in immunoblotting is specifically inhibited with the ASAP1 immunizing peptide.

Immunogen

synthetic peptide located near the C-terminus of mouse ASAP1 (amino acids 1128-1147) conjugated to KLH.

Application

Rabbit anti-ASAP1/Centaurin β4 (ED-20) antibody can be used for immunofluorescence (1:1,000 using mouse NIH3T3 fibroblast cells) and western blot (1:2,000 using rat brain and mouse brain extracts) assays. It is also suitable for use in microarray applications.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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