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53654

Sigma-Aldrich

Anti-Rabbit IgG−Abberior® STAR 600 antibody produced in goat

for STED application

Synonym(s):

Abberior® STAR 600-Anti-Rabbit IgG antibody produced in goat

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.45

biological source

goat

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rabbit

availability

available only in USA and Canada

concentration

~1 mg/mL

fluorescence

λex 604 nm; λem 623 nm in PBS, pH 7.4

storage temp.

−20°C

General description

Abberior STAR 600 was developed for STED and confocal microscopy in the orange spectral region. Abberior STAR 600 is highly photostable and bright orange fluorescent dye. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. It can be effectively excited with excitation light between 550 - 610 nm. Abberior STAR 600 can substitute dyes like Atto™ 590, Atto™ 594 or Alexa Fluor™ 594. For STED microscopy, Abberior STAR 600 can be most efficiently used with a STED Laser wavelength between 750 - 800 nm. Abberior STAR 600 performs exceptionally well in combination with Abberior STAR 635P, STAR 635 or STAR RED. Best results are obtained with freshly prepared samples.


Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 604 (PBS pH 7.4), 604 (H2O), 601 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: : 95 000 (PBS pH 7.4), 95 000 (H2O), 100 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,25 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,25 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 623 (PBS pH 7.4), 623 (H2O), 620 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,85 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,2 (PBS pH 7.4)

Suitability

Designed and tested for fluorescent super-resolution microscopy

Analysis Note

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Other Notes

Find more infomation here Abberior®

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Tobias Müller et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 13(8), 1986-2000 (2012-03-01)
For about a decade, superresolution fluorescence microscopy has been advancing steadily, maturing from the proof-of-principle stage to routine application. Of the various techniques, STED (stimulated emission depletion) microscopy was the first to break the diffraction barrier. Today, it is a

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