We have used this great product for years, even prior to the acquisition by Sigma. Would really appreciate the operations improving to have the 10L bag size available, even prior to the pandemic this has been very poorly handled. Lack of bags to fill, failing QC...so we adjust by buying the smaller size at much higher cost and risk failed runs by having to manipulate filling our own bio-bags with numerous 1L size bottles.
14420C
EX-CELL® 420 Serum-Free Medium for Insect Cells
with L-glutamine, sterile-filtered, suitable for insect cell culture
EX-CELL® 420 Serum-Free Medium for Insect Cells
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 500 mL | Available to ship TODAYfromMILWAUKEE | $77.50 | |
| 1000 mL | Available to ship TODAYfromMILWAUKEE | $135.00 |
About This Item
$77.50
Available to ship TODAYDetails
description
for research or for further manufacturing use
Quality Level
sterility
sterile-filtered
product line
EX-CELL®
form
liquid
technique(s)
cell culture | insect: suitable
components
HEPES: no
L-glutamine: yes
glucose: yes
NaHCO3: yes
shipped in
ambient
storage temp.
2-8°C
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Application
EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or serum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 107 cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.
EX-CELL 420 is a complete medium developed and optimized for the serum-free growth of Sf9 and Sf21 insect cell lines. Cells can be subcultured directly into EX-CELL 420 from serum-free or seum-supplemented media without adaptation. Cultures in EX-CELL 420 routinely reach cell densities greater than 1 × 107 cells/mL with greater than 95% viability. Suspension cultures can be maintained, without refeeding, for more than 10 days. Sf9 and Sf21 cells have been carried for more than 20 passages in EX-CELL 420 with no loss of viability. Protein expression and virus production are improved over serum-containing media.
Legal Information
EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Donot freeze. Protect from light. Several components of the medium arelight labile and should not be exposed to light for lengthy periods of time.
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Hideki Yamaji et al.
Journal of bioscience and bioengineering, 95(2), 185-187 (2005-10-20)
When rat protein kinase C-delta (PKC-delta) was produced by Sf9 cells infected with a recombinant baculovirus in shake-flask culture using a serum-containing medium, the intracellular PKC-delta content decreased in the late period while the extracellular PKC-6 markedly increased. During the
Norikatsu Nishikawa et al.
Cytotechnology, 43(1-3), 3-10 (2008-11-13)
The production of beta-galactosidase by Sf9 cells infected with recombinant Autographa californica nucleopolyhedrovirus (AcNPV) was investigated in shake-flask culture using two serum-free basal media: Grace's medium and TNM-FH (Grace's medium supplemented with lactalbumin hydrolysate and yeast extract). At the time
Fabiana R X Batista et al.
Cytotechnology, 49(1), 1-9 (2008-11-13)
Animal cells can be cultured both in basal media supplemented with fetal bovine serum (FBS) and in serum-free media. In this work, the supplementation of Grace's medium with a set of nutrients to reduce FBS requirements in Spodoptera frugiperda (Sf9)
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