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ED2SS

Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt dihydrate

reagent grade, 98.5-101.5% (titration)

Synonym(s):

Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2

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50 G
$43.40
100 G
$73.60
250 G
$137.00
500 G
$184.00
1 KG
$246.00
2.5 KG
$512.00
5 KG
$882.00

$43.40


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50 G
$43.40
100 G
$73.60
250 G
$137.00
500 G
$184.00
1 KG
$246.00
2.5 KG
$512.00
5 KG
$882.00

About This Item

Empirical Formula (Hill Notation):
C10H14N2Na2O8 · 2H2O
CAS Number:
Molecular Weight:
372.24
Beilstein/REAXYS Number:
3900609
EC Number:
UNSPSC Code:
12352107
PubChem Substance ID:
NACRES:
NA.77

$43.40


In StockDetails


Request a Bulk Order

biological source

synthetic (organic)

Quality Level

grade

reagent grade

agency

suitable for SM 4500 - NH3

assay

98.5-101.5% (titration)

form

powder

reaction suitability

reagent type: chelator

mp

248 °C (dec.) (lit.)

solubility

H2O: soluble, clear, colorless

application(s)

agriculture
clinical research

storage temp.

room temp

SMILES string

O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2

InChI key

OVBJJZOQPCKUOR-UHFFFAOYSA-L

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General description

Ethylenediaminetetraacetic acid (EDTA) disodium salt is an aminopolycarboxylic acid and a hexadentate ligand. It chelates metal cations to form a stable octahedral complex. It is commonly added to buffers, solutions, and even soil samples to control the concentrations of metal ions or to remove metal contaminants. Metal chelators play a crucial role in various research applications, such as biochemistry, molecular biology, and analytical chemistry. For example, EDTA is a blood anticoagulant that chelates calcium ions in the blood, this inhibits clots and is routinely used in hematological assays.

Application

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.
Ethylenediaminetetraacetic acid disodium salt dihydrate has been used as a component of the plasma membrane resuspension buffer,[1] inhibitor′s preservative solution (IS)[2] and luciferase activity assay mix.[3]

Features and Benefits

Reagent grade for general lab use

Other Notes

For additional information on our range of Biochemicals, please complete this form.

pictograms

Health hazardExclamation mark

signalword

Warning

hcodes

Hazard Classifications

Acute Tox. 4 Inhalation - STOT RE 2 Inhalation

target_organs

Respiratory Tract

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Measurement of free IGF-I saliva levels: perspectives in the detection of GH/IGF axis in athletes
Antonelli G, et al.
Clinical Biochemistry, 40(8), 545-550 (2007)
Isolation of plasma membrane-associated membranes from rat liver
Suski JM, et al.
Nature Protocols, 9(2), 312-312 (2014)
Conazole fungicides inhibit Leydig cell testosterone secretion and androgen receptor activation in vitro.
Roelofs MJE, et al.
Toxicology Reports, 1, 271-283 (2014)
EDTA chelation therapy for cardiovascular disease: a systematic review
Seely DMR, et al.
BMC Cardiovascular Disorders, 5(1), 32-32 (2005)
Environmental fate and microbial degradation of aminopolycarboxylic acids
Bucheli-Witschel M and Egli T
FEMS Microbiology Reviews, 25(1), 69-106 (2001)

Protocols

Measure luciferase activity using a luminometer assay detecting light emission, with applications in ATP detection and genetic function reporting.

Enzymatic Assay of Superoxide Dismutase

Related Content

This page is intended to make it easier to find the consumables you need based on the analytical method you’re using. Methods included on this page come from the EPA, Standard Methods and ASTM.

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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