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MilliporeSigma

B0770

Brilliant Blue G

≥80% purity (HPLC), powder

Synonym(s):

Acid blue 90, Coomassie Brilliant Blue G

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$57.20

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$181.00

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$544.00

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About This Item

Empirical Formula (Hill Notation):
C47H48N3NaO7S2
CAS Number:
6104-58-1
Molecular Weight:
854.02
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24891535
EC Number:
228-058-4
MDL number:
MFCD00078482
Colour Index Number:
42655
Beilstein/REAXYS Number:
5230822

Key Documents

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Product Name

Brilliant Blue G, pure

InChI key

RWVGQQGBQSJDQV-UHFFFAOYSA-M

InChI

1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56);/q;+1/p-1

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3C)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=CC(\C=C5C)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

assay

≥80.0% (HPLC)

form

powder

technique(s)

microbe id | staining: suitable

color

dark violet

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Show Differences

1 of 4

This Item
B7920B014927816
Brilliant Blue G pure

Sigma-Aldrich

B0770

Brilliant Blue G

Brilliant Blue R pure

Sigma-Aldrich

B7920

Brilliant Blue R

Brilliant Blue R Dye content ~50 %, Technical grade

Sigma-Aldrich

B0149

Brilliant Blue R

Brilliant Blue R 250, suitable for microscopy

Sigma-Aldrich

27816

Brilliant Blue R

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

color

dark violet

color

-

color

-

color

-

form

powder

form

powder

form

powder

form

powder

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

solubility

H2O: 1 mg/mL

solubility

H2O: 1 mg/mL

solubility

H2O: 10 mg/mL

solubility

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

Brilliant Blue G has been used for determining the urine protein concentration.[1] It has been used for the protein band detection after SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis).[2][3] It has been used as a P2X7R (purinergic receptor) antagonist.[4]

Biochem/physiol Actions

Brilliant Blue G is used for staining protein(s) in polyacrylamide gels.[5] It is also a P2X7 purinergic receptor antagonist. The receptor is mainly involved in neuroinflammatory events. In human SOD1G93A amyotrophic lateral sclerosis mouse, administration of brilliant blue G reduces body weight loss. However, it does not affect survival or motor coordination in transgenic mice.[6]
P2X7 purinergic receptor antagonist.

Legal Information

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBR9153
SHBQ0714
SHBP4840
SHBP1550
SHBM1064

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Neuronal Release of Cytokine IL-3 Triggered by Mechanosensitive Autostimulation of the P2X7 Receptor Is Neuroprotective.
Lim JC, et al.
Frontiers in Cellular Neuroscience, 10, 270-270 (2016)
MATE transport of the E. coli-derived genotoxin colibactin.
Mousa JJ, et al.
Nature microbiology, 1, 15009-15009 (2016)
P2X7 antagonism using Brilliant Blue G reduces body weight loss and prolongs survival in female SOD1G93A amyotrophic lateral sclerosis mice.
PeerJ, e3064-e3064 (2017)
Melatonin attenuates hypertension-induced renal injury partially through inhibiting oxidative stress in rats.
Qiao YF, et al.
Molecular Medicine Reports, 13, 21-21 (2016)
Ahmed H
Principles and Reactions of Protein Extraction, Purification, and Characterization (2004)
View All Related Papers

Protocols

Geles de preparación manual para PAGE y SDS-PAGE utilizando los kits de moldeo de gel bis-Tris mPAGE® TurboMix

Resumen de la química del gel de poliacrilamida e instrucciones detalladas para preparar a mano geles de poliacrilamida con los kits de moldeo del gel bis-Tris TurboMix mPAGE®

Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

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