The raffinose fermentation test is based on the fermentation ability of raffinose which results in the production of acid as end products, the pH of the liquid basal medium will drop, and an indicator changes the color to red. Some organisms produce additional CO2 gas which can be made visible with a Durham’s tube. Raffinose can be used to differentiate Enterobacteriaceae, Bifidobacteria and Lactobacilli, Enterococcus faecium and Streptococcus pneumoniae can utilize that sugar.
Application
Raffinose Discs are recommended to detect raffinose fermenting bacteria in food and environmental samples. They find their application in various sectors such as the food and dairy industry, water industry, pharmaceutical laboratory testing, cosmetic industry, environmental and sanitary testing, clinical diagnostic, etc.
Salmonella, with 2,300+ serotypes, causes half of food-borne illnesses, often from dairy, poultry, and eggs.
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