Biological dyes do not ship with protocols for use. There are published procedures for Sudan Black B for cytology that provide a formulation using Phosphate-buffered Phenol. The Atlas of CytoChemistry and Immunohistochemistry of Hematologic Neoplasms by Sun, Li and Yam, ASCP Press, 1985 gives the following formulation:
Dissolve 32 grams of crystal Phenol in 60 ml of absolute Ethanol. Once dissolved, prepare the Phosphate buffer solution. Add 0.6 grams Sodium Phosphate, then add distilled water until the volume reaches 200ml.
The instructions then state to prepare the working solution by adding 60 ml of the alcoholic Sudan Black B stock solution to 40 ml of the Phenol Phosphate buffer.
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25 G
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About This Item
Empirical Formula (Hill Notation):
C29H24N6
CAS Number:
Molecular Weight:
456.54
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
224-087-1
MDL number:
Beilstein/REAXYS Number:
723248
Colour Index Number:
26150
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agency
certified by the BSC
form
powder
technique(s)
microbe id | staining: suitable
color
dark brown to very dark brown
mp
120-124 °C (lit.)
λmax
415 nm (2nd), 598 nm
ε (extinction coefficient)
≥20000 at 596-603 nm in ethanol
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
CC1(C)Nc2cccc3c(ccc(N1)c23)\N=N\c4ccc(\N=N\c5ccccc5)c6ccccc46
InChI
1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+
InChI key
YCUVUDODLRLVIC-VPHDGDOJSA-N
1 of 4
This Item | |||
|---|---|---|---|
| grade certified by the Biological Stain Commission | grade - | grade certified by the Biological Stain Commission | grade certified by the Biological Stain Commission |
| technique(s) microbe id | staining: suitable | technique(s) - | technique(s) - | technique(s) - |
| color dark brown to very dark brown | color - | color - | color - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| form powder | form powder | form powder | form powder |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Application
Sudan Black B has been used in brain BLAQ (Block Lipids and Aldehyde Quench) technique and is used in lipid detection.[1]
Certified as a fat staining using the Churukian variant of the Lillie-Ashburn supersaturated isopropanol method. Sudan III is substituted for the oil red O in the procedure. Burdon′s stain for bacterial lipid.
Biochem/physiol Actions
Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn′t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black.
signalword
Warning
hcodes
Hazard Classifications
Muta. 2
Storage Class
11 - Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
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Effects of feeding different lipid sources on hepatic histopathology features and growth traits of broiler chickens
Tufarelli V, et.al.
Acta Histochemica, 117, 780-783 (2015)
Acetylated Sudan Black B as a More Specific Histochemical Reagent for Lipides
CASSELMAN WGB
Journal of Cell Science, 321- 322 (1954)
Brain BLAQ: Post-hoc thick-section histochemistry for localizing optogenetic constructs in neurons and their distal terminals
Kupferschmidt DA
Frontiers in Neuroanatomy (2015)
John P. Greer
Wintrobe's Clinical Hematology, 1 (2009)
H N Subramaniam et al.
Journal of biochemical and biophysical methods, 21(1), 9-16 (1990-06-01)
The selective staining of neutral lipids in Human Amnion cells in tissue culture was achieved using a particular fraction of the lipid dye, Sudan black B and a standardized staining procedure. The fraction, termed SBB-I, was isolated by thin-layer chromatography.
Articles
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 199664-25G | 04061838763365 |
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How to dissolve Sudan Black B for staining cytology bone marrow smears?
1 answer-
Helpful?
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Can Sudan black used for Immunofluorescence specifically for paraffinized eyeball sectioning?
1 answer-
Sudan Black B is not a fluorescent dye. Sudan Black B has been used to block autofluorescence. The following information is available from the website. Although the reference below is specific to brain tissue, the expectation is that Sudan Black B should be able to be used for a variety of different tissue types. There is no specific information available for paraffin sections of eyeballs.
Sudan Black B treatment reduces autofluorescence and improves the resolution of in situ hybridization-specific fluorescent signals of brain sections.
Histology and histopathology (2010-06-17)
V C Oliveira, R C V Carrara, D L C Simoes, F P Saggioro, C G Carlotti, D T Covas, L Neder
PMID20552552
ABSTRACT
Interference by autofluorescence is one of the major concerns of immunofluorescence analysis of in situ hybridization-based diagnostic assays. We present a useful technique that reduces autofluorescent background without affecting the tissue integrity or direct immunofluorescence signals in brain sections. Using six different protocols, such as ammonia/ethanol, Sudan Black B (SBB) in 70% ethanol, photobleaching with UV light and different combinations of them in both formalin-fixed paraffin-embedded and frozen human brain tissue sections, we have found that tissue treatment of SBB in a concentration of 0.1% in 70% ethanol is the best approach to reduce/eliminate tissue autofluorescence and background, while preserving the specific fluorescence hybridization signals. This strategy is a feasible, non-time consuming method that provides a reasonable compromise between total reduction of the tissue autofluorescence and maintenance of specific fluorescent labels.Helpful?
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How to dissolve Sudan Black B for staining tissue?
1 answer-
Sudan Black B can be dissolved in a variety of different solvents depending upon the intended use. Dissolve 0.7 grams of Sudan Black B in 100 ml of propylene glycol. The solution should be heated to 100° C for a few minutes, stirred constantly, and then filtered for optimal results.
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