ROAHA
Roche
Anti-HA (12CA5)
from mouse IgG2bκ
Synonym(s):
antibody
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About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
12CA5, monoclonal
assay
>90% (HPLC and SDS-PAGE)
form
frozen liquid (11666606001)
lyophilized (11583816001)
packaging
pkg of 200 μg (11583816001)
pkg of 5 mg (11666606001 [1 ml])
manufacturer/tradename
Roche
concentration
5 mg/ml (11666606001)
isotype
IgG2bκ
epitope sequence
YPYDVPDYA
storage temp.
−20°C
Related Categories
General description
Hemagglutinin HA is a dominant antigen present on the influenza viral surface. HA is a homotrimer, in which each monomer is produced as a single polypeptide. This monomer is cleaved into two subunits HA1 and HA2 by the host protease. The HA gene is mapped to segment 4 of influenza B viral genome.
Specificity
Anti-HA (12CA5) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).
Immunogen
Amino acids 98-106 from the human influenza virus hemagglutinin protein.
Application
Use Anti-HA (12CA5) for the detection of native influenza hemagglutinin protein and recombinant HA-tagged proteins using:
- Dot blots
- Immunochemistry
- Immunoprecipitation
- Western blotting
Biochem/physiol Actions
Membrane fusion and receptor-binding action is the key function of hemagglutinin (HA) and it aids in viral entry and infection. HA is an influenza-virus glycoprotein that regulates membrane fusion and receptor-binding functions during viral entry and infection. The virus gains entry into the host cell via endocytosis and successive membrane fusion mediated by the HA antigen. HA plays a crucial role in viral pathogenesis and host response to viral infection.
Quality
Each lot of Anti-HA antibody is tested for immunoreactivity and purity relative to a reference standard.
Preparation Note
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
The following concentrations should be taken as a guideline:
- Dot blot: 0.1 to 1 μg/ml
- Immunfluorescence: 1-10 μg/ml
- Western blot: 0.1 to 1 μg/ml
Reconstitution
11 583 816 001: Add 0.5 ml of PBS to a final concentration of 0.4 mg/ml.
Other Notes
For life science research only. Not for use in diagnostic procedures.
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Storage Class
13 - Non Combustible Solids
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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