NTMIXKB

Roche

KAPA dNTP Mix (10 mM each)

Name: KAPA dNTP Mix (10 mM each)
Brand: ROCHE

Synonym(s):

DNTP, PCR

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About This Item

NACRES:

NA.55

UNSPSC Code:

41106300

This product is not available through MilliporeSigma in United States. Please go to sequencing.roche.com to purchase directly.

Key Documents

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grade

Molecular Biology

assay

>99% (HPLC)

form

liquid

shelf life

≤18 mo.

packaging

pkg of 5 mL (KK1041)
pkg of 500 μL (KK1017)

manufacturer/tradename

Roche

impurities

Bacterial contamination assay, passes test
DNase, none detected
Endonuclease contamination assay, passes test
Exonuclease contamination assay, passes test
Human contamination assay, passes test
RNase, none detected
nicking activity, none detected
phosphatase, none detected
protease, none detected

color

colorless

solubility

water: miscible

shipped in

dry ice

storage temp.

−20°C

Quality Level

100

Analysis Note

KAPA dNTPs are >99% pure by HPLC and are free of DNase, RNase, protease, phosphatase, and nicking activity. All kit components are subjected to stringent functional quality control, and meet strict requirements with respect to DNA contamination.

Application

KAPA dNTP Mix has been used in:

Standard PCR[1][2][3]
High fidelity PCR
Quantitative PCR
End-repair or A-tailing
cDNA synthesis
DNA sequencing
DNA labeling
Microarrays

KAPA dNTPs are suitable for use in any molecular biology application requiring high-quality dNTPs.

Features and Benefits

KAPA dNTPs are available as individual dNTPs or as a ready-to-use dNTP Mix.
KAPA dNTP Mix is supplied with KAPA Taq, KAPA2G Fast, KAPA2G Robust, KAPA LongRange and KAPA HiFi PCR Kits.
KAPA dNTPs are subjected to strict quality control to ensure high performance, particularly with B-family polymerases that are sensitive to poisoning by uracil.
Compatible with any commercially available DNA polymerases.

General description

KAPA dinucleotide triphosphates (dNTPs) provide high-quality, molecular, premium grade dinucleotide phosphates (dNTPs) mainly used for polymerase chain reaction (PCR).

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Preparation Note

Always ensure that the product KAPA dNTP Mix has been fully thawed and mixed before use. KAPA dNTPs may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that the sample has been handled carefully and not contaminated, the samples are not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that storing the reagents at temperatures above -20°C makes them more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

does not flash

flash_point_c

does not flash

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