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X-tremeGENE^™ 9 DNA Transfection Reagent

Name: X-tremeGENE<SUP>™</SUP> 9 DNA Transfection Reagent
Brand: ROCHE
Price: 284 USD
Availability: InStock

Polymer reagent for transfecting common cell lines

Synonym(s):

transfection reagent

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Pack Size	Availability	Price
0.4 mL	Available to ship TODAYfromMILWAUKEE	$284.00
1 mL	Available to ship TODAYfromMILWAUKEE	$551.00
5 x 1 mL	Available to ship TODAYfromMILWAUKEE	$2,420.00

About This Item

NACRES:

NA.55

UNSPSC Code:

41106502

$284.00

Available to ship TODAYDetails

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grade

Molecular Biology

form

liquid (aqueous solution)

usage

mL (suitable for 165 transfections)

packaging

pkg of 0.4 mL (06365779001), pkg of 1.0 mL (06365787001), pkg of 5 × 1.0 mL (06365809001)

manufacturer/tradename

Roche

technique(s)

transfection: suitable

storage temp.

2-8°C

Related Categories

Cloning & Expression

Roche Life Science Products

Transfection Reagents

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This Item	XTGHP-RO	XTG360-RO	SITRAN-RO
Roche XTG9-RO X-tremeGENE^™ 9 DNA Transfection Reagent	Roche XTGHP-RO X-tremeGENE^™ HP DNA Transfection Reagent	Roche XTG360-RO X-tremeGENE^™ 360 Transfection Reagent	Roche SITRAN-RO X-tremeGENE^™ siRNA Transfection Reagent
grade Molecular Biology	grade Molecular Biology	grade Molecular Biology	grade Molecular Biology
technique(s) transfection: suitable	technique(s) transfection: suitable	technique(s) transfection: suitable	technique(s) transfection: suitable
packaging pkg of 0.4 mL (06365779001), pkg of 5 × 1.0 mL (06365809001), pkg of 1.0 mL (06365787001)	packaging pkg of 0.4 mL (06366244001), pkg of 1.0 mL (06366236001), pkg of 5 × 1 mL (06366546001)	packaging pkg of 0.4 mL (08724105001), pkg of 1.0 mL (08724121001), pkg of 5 × 1.0 mL (08724156001)	packaging pkg of 1 mL (04476093001 [1 mg/ml]), pkg of 5 × 1 mL (04476115001 [5 x 1 mg/ml])
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C
manufacturer/tradename Roche	manufacturer/tradename Roche	manufacturer/tradename Roche	manufacturer/tradename Roche
form liquid (aqueous solution)	form liquid (aqueous solution)	form liquid (aqueous solution)	form -

General description

X-tremeGENE 9 DNA Transfection Reagent is a proprietary blend of lipids and other components supplied in 80% ethanol, filtered through 0.2 μm pore size membrane, and packaged in glass vials.

Application

X-tremeGENE^™ 9 DNA Transfection Reagent is a non-liposomal multi-component reagent for experiments involving cellular analysis. Due to its extremely low cytotoxicity, minimal need for optimization, and the ability to provide high transfection efficiency in a wide range of commonly used cell lines even in the presence of serum, it is well suited for applications in all fields of cellular analysis.

X-tremeGENE^™ 9 DNA Transfection Reagent is well suited for cellular analysis applications such as:

Expression of recombinant proteins for functional analysis.
Physiological studies of metabolic pathways.[1][2]
Analysis of regulatory sequences using reporter gene assays.[3]
Gene expression assays.[3][4]
Cancer research studies.[5]
Target evaluation.

Features and Benefits

Generate physiologically relevant results using a reagent with extremely low cytotoxicity for maximum post-transfection cell viability.
Save time and eliminate multiple handling steps; simply dilute X-tremeGENE 9 DNA Transfection Reagent, incubate with plasmid DNA, and pipet the mixture directly onto your cells (with or without serum).
Avoid time-consuming optimization procedures in commonly used cell lines.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE 9 DNA Transfection Reagent can be used to perform up to 6,600 transfections in 96-well plates using 3:1 ratio and up to 10,000 transfections using 2:1 ratio.

Analysis Note

Each lot of X-tremeGENE^™ 9 DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE^™ 9 DNA Transfection Reagent (ratio 3:1 μl/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

X-tremeGENE is a trademark of Roche

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pictograms

GHS02,GHS07

signalword

Danger

pcodes

P210 - P233 - P280 - P303 + P361 + P353 - P337 + P313 - P370 + P378

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

334.4 °F

flash_point_c

168 °C

hcodes

H225,H319

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Compartmental expression of trkB receptor protein in the developing striatum.

L C Costantini et al.

Neuroscience, 89(2), 505-513 (1999-03-17)

To investigate the role of neurotrophins in the initial formation of striatal patch versus matrix, the spatial and temporal expression of trkB receptors was examined using immunohistochemistry. Polyclonal antibodies, against the C-terminus or the tyrosine kinase domain, revealed trkB-immunoreactive cells

Involvement of O-glycosylation defining oncofetal fibronectin in epithelial-mesenchymal transition process.

Leonardo Freire-de-Lima et al.

Proceedings of the National Academy of Sciences of the United States of America, 108(43), 17690-17695 (2011-10-19)

The process termed "epithelial-mesenchymal transition" (EMT) was originally discovered in ontogenic development, and has been shown to be one of the key steps in tumor cell progression and metastasis. Recently, we showed that the expression of some glycosphingolipids (GSLs) is

TTS mapping: integrative WEB tool for analysis of triplex formation target DNA sequences, G-quadruplets and non-protein coding regulatory DNA elements in the human genome.

Piroon Jenjaroenpun et al.

BMC genomics, 10 Suppl 3, S9-S9 (2010-01-09)

DNA triplexes can naturally occur, co-localize and interact with many other regulatory DNA elements (e.g. G-quadruplex (G4) DNA motifs), specific DNA-binding proteins (e.g. transcription factors (TFs)), and micro-RNA (miRNA) precursors. Specific genome localizations of triplex target DNA sites (TTSs) may

Synergistic effects of ascorbate and sorafenib in hepatocellular carcinoma: New insights into ascorbate cytotoxicity.

Lauren Rouleau et al.

Free radical biology & medicine, 95, 308-322 (2016-04-03)

We investigated the mechanism of selective ascorbate-induced cytotoxicity in tumor cells, including Hep G2 cells, compared to primary hepatocytes. H2O2 formation was required for ascorbate cytotoxicity, as extracellular catalase treatment protected tumor cells. H2O2 generated by glucose oxidase treatment also

Splicing factor SRSF1 negatively regulates alternative splicing of MDM2 under damage.

Daniel F Comiskey et al.

Nucleic acids research, 43(8), 4202-4218 (2015-04-08)

Genotoxic stress induces alternative splicing of the oncogene MDM2 generating MDM2-ALT1, an isoform attributed with tumorigenic properties. However, the mechanisms underlying this event remain unclear. Here we explore MDM2 splicing regulation by utilizing a novel minigene that mimics endogenous MDM2

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Articles

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

X-tremeGENE™ siRNA Transfection Reagent

Small inhibitory RNAs offer easy gene expression knockdown in mammalian cells, revolutionizing gene research.

Reverse Transfection of Plasmid DNA

Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

How Transfection Works

This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.

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Protocols

Co-transfection of Plasmid DNA

Transient co-transfection of plasmids for cellular studies in protein interaction, transcription factor, and gene knockdown analyses.

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

X-tremeGENE™ HP DNA Transfection Reagent Protocol

Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents

Global Trade Item Number

SKU	GTIN
6365809001	04061832722764
6365787001	04061832722740
6365779001	04061837684791

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