Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

View All Documentation

11759779001

Roche

BM Chemiluminescence ELISA Substrate (AP)

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352204

usage

sufficient for 1,000 tests (ELISA)
 sufficient for 600 tests (SEAP)

packaging

pkg of 150 mL

manufacturer/tradename

Roche

shipped in

wet ice

storage temp.

2-8°C

Specificity

The AP assay system is designed for measuring alkaline phosphatase of various sources (e.g. intestine, placenta, milk, bacteria).

Features and Benefits

  • Sensitive: Approximately 10fg intestinal alkaline phosphatase can be detected
  • High dynamic measuring range: Linear range of more than five orders of magnitude
  • Constant light emission: The assay produces a long-lasting light emission instead of a short peak kinetics

Components

1. Alkaline Phosphatase Substrate, CSPD
2. Enhancer, Emerald II
3. Assay Buffer

Principle

CSPD is dephosphorylated by alkaline phosphatase (AP). The resulting instable dioxetane anion decomposes and emits light at a maximum wavelength of 477nm. Chemiluminescence-enhancing reagents improve the quantum yield of the excited state by more than 500 fold.

Preparation Note

Working solution: Preparation of working solution

The substrate reagent is composed of AP-substrate (bottle 1), Enhancer (bottle 2) and Assay Buffer (bottle 3). Prepare substrate reagent freshly before use. The ratio of the components depends on the application. The substrate reagent is stable at least for one day at 2 to 8 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
32311800
21762200
11498300
11200200
13995020

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Martijn J Wilmer et al.
Cell and tissue research, 339(2), 449-457 (2009-11-11)
Reabsorption of filtered solutes from the glomerular filtrate and excretion of waste products and xenobiotics are the main functions of the renal proximal tubular (PT) epithelium. A human PT cell line expressing a range of functional transporters would help to
JunBae Jee et al.
Journal of clinical microbiology, 42(12), 5664-5672 (2004-12-08)
We investigated the acquisition and prevalence of Chlamydophila sp. infection in calves. Specimens were collected at weekly intervals from birth to week 12 postpartum from 40 female Holstein calf-dam pairs in a dairy herd. Real-time PCR detected, quantified, and differentiated

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service