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WBAVDP001

Immobilon® Block - PO (Phosphoprotein Blocker)

Western blotting, dot/slot blotting, mass spectrometry, phosphorolation assay

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About This Item

UNSPSC Code:
41116010
NACRES:
NB.22
eCl@ss:
42029053
Pricing and availability is not currently available.
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Quality Level

packaging

pkg of 500 mL

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

membrane area

1000 cm2 , membrane coverage

detection method

chemiluminescent (phosphoprotein), fluorometric (phosphoprotein)

shipped in

ambient

storage temp.

15-25°C

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This Item
WBAVDFL01WBAVDCH01182877
technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

-

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

100

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

-

membrane area

1000 cm2 , membrane coverage

membrane area

1000 cm2 , membrane coverage

membrane area

1000 cm2 , membrane coverage

membrane area

-

packaging

pkg of 500 mL

packaging

pkg of 500 mL

packaging

pkg of 500 mL

packaging

-

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

-

Preparation Note

Stable for 1 year at 20–25°C

Analysis Note

For optimum results, use Immobilon-P membrane for chemiluminescence (luminol) or visual detection (e.g. TMB), and Immobilon-FL membrane for fluorescent detection applications.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Johnathan M Borland et al.
International journal of molecular sciences, 24(2) (2023-01-22)
Like many social behaviors, aggression can be rewarding, leading to behavioral plasticity. One outcome of reward-induced aggression is the long-term increase in the speed in which future aggression-based encounters is initiated. This form of aggression impacts dendritic structure and excitatory
H N Gao et al.
Journal of dairy science, 100(9), 7696-7709 (2017-06-26)
The ratio of different AA in the diets of cows is vital to improve milk protein yield. β-Casein is one of the important milk proteins with high nutritive value. However, the suitable ratio of essential amino acids (EAA) for the
Vanesa D Ramseyer et al.
American journal of physiology. Renal physiology, 308(2), F149-F156 (2014-11-08)
Thick ascending limbs reabsorb 30% of the filtered NaCl load. Nitric oxide (NO) produced by NO synthase 3 (NOS3) inhibits NaCl transport by this segment. In contrast, chronic angiotensin II (ANG II) infusion increases net thick ascending limb transport. NOS3
I-Ting Chen et al.
EMBO reports, 22(12), e52254-e52254 (2021-10-12)
Promyelocytic leukemia protein (PML) is a tumor suppressor possessing multiple modes of action, including induction of apoptosis. We unexpectedly find that PML promotes necroptosis in addition to apoptosis, with Pml-/- macrophages being more resistant to TNF-mediated necroptosis than wild-type counterparts
Riasat Zaman et al.
The Journal of cell biology, 220(6) (2021-04-10)
Activated ezrin-radixin-moesin (ERM) proteins link the plasma membrane to the actin cytoskeleton to generate apical structures, including microvilli. Among many kinases implicated in ERM activation are the homologues LOK and SLK. CRISPR/Cas9 was used to knock out all ERM proteins

Articles

The possible causes and potential remedies for challenges encountered as a result of blocking, washing, antibody incubation, and detection/exposure of Western blots

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We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.

Lo entendemos. Los Westerns pueden ser un dolor de muelas, y ciertamente hemos hecho nuestra parte. Así que, ¡comparte con nosotros tus feos borrones y recibe una sorpresa! Y aunque no podemos decirte de qué se trata -eso estropearía la sorpresa-, salvo que despertará la creatividad en el laboratorio.

Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.

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Global Trade Item Number

SKUGTIN
WBAVDP00104053252616556

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