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Immobilon^® Block - FL (Fluorescent Blocker)

Name: Immobilon<SUP>®</SUP> Block - FL (Fluorescent Blocker)
Brand: MM

Fluorescent Detection for Western blotting, dot/slot blotting, mass spectrometry and fluorescence assays

Synonym(s):

Fluorescent Blocker, Immobilon Blocking Reagent

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About This Item

UNSPSC Code:

41116010

NACRES:

NB.22

eCl@ss:

42029053

Pricing and availability is not currently available.

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Quality Level

300

packaging

pkg of 500 mL

manufacturer/tradename

Immobilon^®

technique(s)

western blot: suitable

membrane area

1000 cm² , membrane coverage

detection method

fluorometric

shipped in

ambient

storage temp.

15-25°C

Related Categories

Immunodetection Reagents & Substrates

Protein Electrophoresis & Western Blotting

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This Item	W0138	IPFL00010	IPFL20200
Millipore WBAVDFL01 Immobilon^® Block - FL (Fluorescent Blocker)	Sigma-Aldrich W0138 Western Blocker^™ Solution	Millipore IPFL00010 Immobilon^®-FL PVDF Membrane	Millipore IPFL20200 Immobilon^®-FL PVDF Membrane
packaging pkg of 500 mL	packaging -	packaging -	packaging -
manufacturer/tradename Immobilon^®	manufacturer/tradename -	manufacturer/tradename Immobilon^®	manufacturer/tradename Immobilon^®
technique(s) western blot: suitable	technique(s) -	technique(s) dot blot: suitable, western blot: suitable	technique(s) dot blot: suitable, western blot: suitable
membrane area 1000 cm² , membrane coverage	membrane area -	membrane area -	membrane area -
detection method fluorometric	detection method -	detection method chemiluminescent, colorimetric, fluorometric	detection method chemiluminescent, colorimetric, fluorometric
shipped in ambient	shipped in -	shipped in ambient	shipped in ambient

General description

Immobilon^® Block Noise Cancelling Reagents are designed especially for Western blotting applications. This family of protein-free, ready-to-use buffers has been optimized to reduce background levels when using chemiluminescence, fluorescent, or phosphotyrosine detection. Bløk buffers are ideal for use with Immobilon-P Western blotting membranes and the SNAP i.d. system, and are also compatible with standard Western blotting techniques.

Application

For use on immunoblots.

Packaging

Sufficient reagent for 19 mini blots (8x7 cm)

Preparation Note

Stable for 2 years at 20–25°C

Analysis Note

For optimum results, use Immobilon-P membrane for chemiluminescence (luminol) or visual detection (e.g. TMB), and Immobilon-FL membrane for fluorescent detection applications.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

flash_point_f

does not flash

flash_point_c

does not flash

Storage Class

12 - Non Combustible Liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Huntingtin phosphorylation governs BDNF homeostasis and improves the phenotype of Mecp2 knockout mice.

Yann Ehinger et al.

EMBO molecular medicine, 12(2), e10889-e10889 (2020-01-09)

Mutations in the X-linked MECP2 gene are responsible for Rett syndrome (RTT), a severe neurological disorder for which there is no treatment. Several studies have linked the loss of MeCP2 function to alterations of brain-derived neurotrophic factor (BDNF) levels, but

We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.

Replanteamiento de Western blotting con herramientas y estrategias para optimizar sus Western blots

Lo entendemos. Los Westerns pueden ser un dolor de muelas, y ciertamente hemos hecho nuestra parte. Así que, ¡comparte con nosotros tus feos borrones y recibe una sorpresa! Y aunque no podemos decirte de qué se trata -eso estropearía la sorpresa-, salvo que despertará la creatividad en el laboratorio.

Brochure-Rethink Western Blotting

Antibody recovery and reuse in the SNAP i.d.®2.0 immunodetection system

Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.

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Global Trade Item Number

SKU	GTIN
WBAVDFL01	04053252464478

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