Skip to Content
MilliporeSigma
All Photos(1)

Documents

69066-M

Millipore

Recombinant Enterokinase

Synonym(s):

Enterokinase Enzyme, Enterokinase Protein, Recombinant Enterokinase

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352202
NACRES:
NA.54

biological source

bovine

Quality Level

200

recombinant

expressed in E. coli

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

technique(s)

targeted protein degradation: suitable

suitability

suitable for enzyme test

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

General description

Recombinant Enterokinase (rEK) is a highly purified preparation of the catalytic subunit of bovine enterokinase, a serine protease which recognizes the identical cleavage site as the native enzyme (AspAspAspAspLys↓) and has similar enzymatic activity. This enzyme is produced in E. coli and purified to yield the highest activity available, and is qualified for specific cleavage of appropriate fusion proteins. Supplied as a solution in 50% glycerol. EC 3.4.21.9, M.W. 26,300.

Components

•50 URecombinant Enterokinase

•2 ml1X rEK Dilution/Storage Buffer

•1 ml10X rEK Cleavage Buffer

•10 µgCleavage Control Protein

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Unit Definition

One unit is defined as the amount of enzyme that will cleave 50 µg of fusion protein in 16 hours at 23°C, in a buffer containing 20 mM Tris-HCl pH 7.4, 50 mM NaCl, 2 mM CaCl₂.

Other Notes

Collins-Racie, L.A. et al. 1995. Bio/Technology 13, 982.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Cleavage Enzymes for Fusion Protein Purification

Find highly-specific cleavage enzymes for isolating proteins during fusion protein purification including enterokinase, factor Xa, HRV 3C protease, and thrombin.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service