1.52062
Chromolith® RP-18 endcapped (monolithic) HPLC Columns
L × I.D. 50 mm x 2 mm HPLC Columns Validation Kit (3 Columns from 3 different batches)
Synonym(s):
Chromolith® RP-18 endcapped 50-2 HPLC columns Validation Kit (3 columns from 3 different batches)
About This Item
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product name
Chromolith® RP-18 endcapped, L × I.D. 50 mm x 2 mm HPLC columns Validation Kit (3 columns from 3 different batches)
material
PEEK column
Quality Level
agency
suitable for USP L1
product line
Chromolith®
feature
endcapped
packaging
pkg of 3 ea
extent of labeling
18% Carbon loading
parameter
200 bar pressure
45 °C max. temp.
technique(s)
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)
column L × I.D.
5 cm × 2 mm
matrix
monolithic silica
matrix active group
C18 bonding phase
pore size
1.5 μm macroporosity
13 nm mesoporosity
operating pH range
2-7.5
separation technique
reversed phase
storage temp.
no temp limit
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General description
Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.
Chromolith® RP-18 endcapped 50-2 columns have macropores with 1.5 μm diameter, giving a column efficiency exceeding 100,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® RP-18 endcapped 50-2 columns fall under the USP L1 Classification. The column efficiency is comparable to a 3-4 μm particle size fully porous particulate column. Chromolith® RP-18 endcapped 50-2 columns are perfectly suitable for LC-MS applications.
The column dimension of 50 mm length and 2 mm I.D. is the best choice for fast separations on any U/HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® RP-18 endcapped 5-2 guard cartridges (3 pieces) [1.52009] with the Chromolith® Guard Cartridge Holder (kit) 5 mm x 2 mm [1.52004]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Legal Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
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