Skip to Content
MilliporeSigma

1.44154

PyroDetect Kit

Reagents and Interleukin-1 ELISAβ kit for Monocyte Activation test (MAT), for Pyrogen testing

Synonym(s):

Pyrogen testing kit

Slide 1 of 1
Sign In to View Organizational & Contract Pricing.

Select a Size

1 VIAL

$1,730.00

$1,730.00

Check Cart for Availability
Request more information

About This Item

NACRES:
NA.24
UNSPSC Code:
41116129
Agency:
EP Chapter 2.6.30
Suitability:
pyrogenic bacteria
Manufacturer/tradename:
PyroDetect
Application(s):
pharmaceutical
pyrogen testing
Storage temp.:
2-8°C

Key Documents

View All Documentation

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

agency

EP Chapter 2.6.30

feature

ready-to-use

packaging

pkg of 1 kit

manufacturer/tradename

PyroDetect

application(s)

pharmaceutical
pyrogen testing

compatibility

configured for PyroDetect Systems

shipped in

ambient

storage temp.

2-8°C

suitability

pyrogenic bacteria

Quality Level

200

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
1.44158LY0100PYR0MATKIT
PyroDetect Kit Reagents and Interleukin-1 ELISAβ kit for Monocyte Activation test (MAT), for Pyrogen testing

Millipore

1.44154

PyroDetect Kit

PyroDetect Interleukin Standard Interleukin-1Beta standard for use in the Monocyte Activation Test (MAT) for Pyrogen testing

Millipore

1.44158

PyroDetect Interleukin Standard

Lysozyme Activity Kit sufficient for 100 tests

Sigma-Aldrich

LY0100

Lysozyme Activity Kit

PyroMAT® kit includes Interleukin IL-6 Standard and Interleukin-6ELISA kit, suitable for (suitable for Monocyte Activation Test (MAT)), suitable for pyrogen testing

Millipore

PYR0MATKIT

PyroMAT® kit

suitability

pyrogenic bacteria

suitability

pyrogenic bacteria

suitability

-

suitability

pyrogenic bacteria

manufacturer/tradename

PyroDetect

manufacturer/tradename

PyroDetect

manufacturer/tradename

-

manufacturer/tradename

PyroMAT®

compatibility

configured for PyroDetect Systems

compatibility

configured for PyroDetect System

compatibility

-

compatibility

configured for PyroMAT®

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

400

agency

EP Chapter 2.6.30

agency

-

agency

-

agency

EP Chapter 2.6.30

application(s)

pharmaceutical
pyrogen testing

application(s)

pharmaceutical
pyrogen testing

application(s)

-

application(s)

pharmaceutical
pyrogen testing

General description

PyroDetect Kit is a Monocyte Activation Test (MAT) for the detection of pyrogens. This test allows the determination of the pyrogenic activity in a solution or suspension by inducing interleukin production in cell cultures with human monocytes.

Application

The PyroDetect Kit is used for the detection and quantification of the pyrogenic activity in pharmaceutical products to ensure their safety.

Biochem/physiol Actions

The PyroDetect System uses cryopreserved human whole blood as a source of monocytes. The response to pyrogenic substances is determined by measurement of the Interleukin-1β (IL-1β) production. The ELISA (enzyme-linked immunosorbent assay) microplate in the PyroDetect System is precoated with an antibody specific for IL-1β. Interleukin molecules present in the culture supernatant, transferred from the blood cultures, are bound to the immobilized antibody.
The unbound substances are washed away, and an enzyme-linked polyclonal antibody specific for IL-1β is added. The IL-1β bound complex is then detected in a color reaction initiated by the addition of a suitable substrate. The efficiency of IL-1β production in the pyrogen reaction is calculated from the data of a photometrical quantification of the colored product by the enzymatic reaction in an ELISA reader.

Features and Benefits

  • Ready-to-use kit
  • Greater product safety
  • Highly compatible
  • In vitro test with cryoblood
  • Reliable results
  • Regulatory recognition

Other Notes

Each PyroDetect Kit contains:
  • Cell Culture Microplate for 96 microcultures, with lid - 1 plate
  • Cell Culture Medium - RPMI with L-Glutamine and HEPES - 50 mL
  • IL-1β Microplate - 12 strips of 8 wells each, coated with a monoclonal antibody specific for IL-1β - 1 plate
  • IL-1β Conjugate
  • Wash Buffer Concentrate
  • Color Reagent Α
  • Color Reagent Β
  • Stop Solution
PyroDetect Cryoblood (1.44155)
Reference Standard Endotoxin (1.44161)
NEP Control of HKSA (MATHKSA)
NEP Control of Flagellin (MATFLAGELLIN)

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Warning

Hazard Classifications

Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1

Storage Class

8A - Combustible, corrosive hazardous materials

wgk

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service