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Chromolith® Si (monolithic) HPLC Columns

L × I.D. 100 mm x 25 mm Prep Column

Synonym(s):

Chromolith® Prep Si 100-25 column

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

Chromolith® Si, L × I.D. 100 mm x 25 mm Prep column

material

PEEK column

Quality Level

100

agency

suitable for USP L3

product line

Chromolith®

feature

endcapped: no

packaging

1 ea of

parameter

100 bar pressure
45 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable (Prep LC)
UHPLC-MS: suitable
UHPLC: suitable

column L × I.D.

10 cm × 2 mm

matrix

monolithic silica

matrix active group

Silica bonding phase

pore size

12 nm mesoporosity
3 μm macroporosity

operating pH range

2-7.5

separation technique

hydrophilic interaction (HILIC)
normal phase

storage temp.

no temp limit

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General description

Preparative HPLC involves much higher sample loading volumes than analytical chromatography. Consequently, greater sample throughput and separation speed are essential for optimal productivity. These criteria are best fulfilled by Chromolith® Prep Si columns. The combination of macro and mesopores maximizes separation efficiency and flow rate, while minimizing resistance.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Analysis Note

Pressure: ≤ 15 bar
Theoretical Plates (N/m) (2-nitroanisol): ≥ 50000
Capacity factor (2-nitroanisol): 1.0 - 2.0
Tailing (USP): 0.90 - 1.45 %
separation factor (2-nitroanisol/nitrobenzene): 3.2 - 4.0

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

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