Skip to Content
MilliporeSigma
All Photos(1)

Documents

1.15444

Sigma-Aldrich

Coomassie Brilliant blue G 250 (C.I. 42655)

for electrophoresis

Synonym(s):

Coomassie Brilliant blue G 250 (C.I. 42655), Acid blue 90, Brilliant Blue G, Cyanine G, Polar Blue G, SERVA BLUE G, protein gel stain

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C47H48N3NaO7S2
CAS Number:
6104-58-1
Molecular Weight:
854.02
MDL number:
MFCD00078482
UNSPSC Code:
41105305
EC Index Number:
228-058-4
NACRES:
NC.01

Quality Level

100

potency

>5000 mg/kg LD50, oral (Rat)

technique(s)

protein staining: suitable

pH

6.4 (20 °C, 10 g/L in H2O)

solubility

40 g/L

bulk density

520 kg/m3

storage temp.

no temp limit

InChI

1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30,48H,6-8,31-32H2,1-5H3,(H-,51,52,53,54,55,56);/q;+1/p-1

InChI key

CMGWKYXJIYYESN-UHFFFAOYSA-M

Related Categories

General description

Coomassie Brilliant blue G 250 (C.I. 42655) is a member of Coomassie brilliant blue (CBB), which is a triphenyl methane dye. CBB dyes are widely used for the visualization of proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. CBB G-250 (greenish tinted blue) dye is preferably used in Bradford assay for the quantification of proteins.

Application

Coomassie Brilliant blue G 250 (C.I. 42655) has been used to stain gel in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. It has also been used in Bradford protein assay.

Analysis Note

Identity (UV/VIS-Spectrum): passes test
Absorption maximum λmax. (buffer pH 7.0): 577 - 584 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; buffer pH 7.0; calc. on dried substance): 450 - 570
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for electrophoresis: passes test

Legal Information

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Robert G E Krause et al.
Analytical biochemistry, 566, 107-115 (2018-11-21)
Coomassie brilliant blue R250, an anionic dye is the most popular stain to detect proteins resolved in SDS-PAGE gels. Crystal violet, a cationic dye was found to be versatile and stained proteins in SDS-PAGE gels and in zymograms. Stained proteins
Nazim Mekaoui et al.
Journal of chromatography. A, 1232, 134-141 (2011-12-22)
Commercial samples of Coomassie Brilliant Blue G-250 (CBB) were not pure enough to give reliable results when used as indicator of amine content in biological material. The polar and apolar impurities produce unacceptable biases in the results. Counter current chromatography
Gang Lu et al.
Bio-protocol, 10(6), e3565-e3565 (2021-03-05)
Although many spherical and rod-shaped plant virus purification protocols are now available, only a few protocols on filamentous plant virus purification have been published. Here, we report a protocol for large-scale purification of Rice stripe virus (RSV) from RSV-infected rice
Mohammad-Bagher Ebrahim-Habibi et al.
Scientific reports, 9(1), 1273-1273 (2019-02-06)
Investigation of non-covalent interaction of hydrophobic surfaces with the protein G (PrG) is necessary due to their frequent utilization in immunosensors and ELISA. It has been confirmed that surfaces, including carbonous-nanostructures (CNS) could orient proteins for a better activation. Herein

Articles

Sample Prep & Gel Electrophoresis Troubleshooting

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service