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03-0160-00

SMC® Human IL-1β High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human IL-1β in human serum and plasma

Synonym(s):

IL-1 beta Single Molecule Counting Kit, IL-1 beta immunoassay kit, IL-1 beta ultra-sensitive immunoassay, Interleukin-1 beta immunoassay kit

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
Species reactivity:
human
Technique(s):
single molecule counting: suitable
Usage:
 kit sufficient for 96 wells
Compatibility:
for use with FemtoQuest and SMCxPRO® instruments
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Product Name

SMC® Human IL-1β High Sensitivity Immunoassay Kit,

description

Used for ultrasensitive quantification of Human IL-1β in human serum and plasma

product line

SMC®

usage

 kit sufficient for 96 wells

species reactivity

human

technique(s)

single molecule counting: suitable

input

plasma (K2 EDTA)
serum

UniProt accession no.

compatibility

for use with FemtoQuest and SMCxPRO® instruments

shipped in

wet ice

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This Item
03-0167-0003-0190-0003-0164-00
technique(s)

ELISA: suitable, single molecule counting: suitable

technique(s)

ELISA: suitable, single molecule counting: suitable

technique(s)

single molecule counting: suitable

technique(s)

ELISA: suitable, single molecule counting: suitable

description

0.05-0.4 pg/mL, refer to CoA for lot-specific LLOQ

description

0.06-0.23 pg/mL, refer to CoA for lot-specific LLOQ

description

0.04-0.17 pg/mL, refer to CoA for lot-specific LLOQ

description

0.1-0.4 pg/mL, refer to CoA for lot-specific LLOQ

usage

1 kit sufficient for 96 wells

usage

1 kit sufficient for 96 wells

usage

-

usage

1 kit sufficient for 96 wells

species reactivity

human

species reactivity

human

species reactivity

mouse, feline, rat

species reactivity

human

manufacturer/tradename

SMC®

manufacturer/tradename

SMC®

manufacturer/tradename

SMC®

manufacturer/tradename

SMC®

assay range

sensitivity: 0.20 pg/mL
(lower limit of quantification)

assay range

sensitivity: 0.12 pg/mL
(lower limit of quantification), inter-assay cv: <20%
intra-assay cv: <15%
standard curve range: 0.06-60 pg/mL

assay range

sensitivity: 0.09 pg/mL
(Lower Limit of Quantification), inter-assay cv: 7%
intra-assay cv: 4%
(five normal plasma samples run in triplicate by 3 different operators on 3 different days.)

assay range

sensitivity: 0.20 pg/mL
(lower limit of quantification), inter-assay cv: <15%
intra-assay cv: <10%
standard curve range: 0.10-100 pg/mL

General description

Interleukin-1 beta (IL-1β) is a pro-inflammatory cytokine produced mainly by activated macrophages and other immune cells. It plays a pivotal role in mediating inflammatory responses, promoting the activation of immune cells, and influencing the production of other cytokines. IL-1β is also involved in various physiological processes, including fever induction and the regulation of immune system responses, making it a key player in both health and disease.

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Features and Benefits

  • High-Performance Sensitivity: The SMC®technology excels in the quantification of IL-1β in human serum and plasma, enabling highly accurate measurements.
  • Built for Confidence: With low variability and high reproducibility, you can trust your data across experiments. Whether you′re profiling inflammation or validating biomarkers, this assay delivers consistent, high-quality results.
  • Deeper Insights into Inflammation: IL-1β is a key player in autoimmune diseases, neuroinflammation, and sepsis. This assay helps you explore its role with unmatched clarity.
  • Early Biomarker Detection: The ultra-sensitive performance supports early-stage discovery and therapeutic development.

Packaging

96 well assay

Analysis Note

  • Sensitivity: LLOQ 200 fg/mL* (SMCxPRO® Instrument)
  • Standard Curve Range: 50-50,000 fg/mL
  • Intra-assay CV: <15%
  • Inter-assay CV: <20%
  • Linearity: Serum: 107% Plasma: 111%
  • Recovery: Serum: 85% Plasma: 83%

*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although IL-1β was detectable in non-human primate, rat, equine, feline and canine samples, the assay has not been verified for these species.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
03-0160-0004054839615801

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