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408727

Sigma-Aldrich

Polyethylenimine, branched

average Mw ~25,000 by LS, average Mn ~10,000 by GPC, branched

Synonym(s):

aziridine, homopolymer, PEI

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100 ML
$133.00
250 ML
$263.00
1 L
$664.00

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100 ML
$133.00
250 ML
$263.00
1 L
$664.00

About This Item

Linear Formula:
H(NHCH2CH2)nNH2
CAS Number:
9002-98-6
MDL number:
MFCD00084427
UNSPSC Code:
12162002
PubChem Substance ID:
24865591
NACRES:
NA.23

$133.00

In StockDetails

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Quality Level

200

form

viscous liquid

mol wt

average Mn ~10,000 by GPC
average Mw ~25,000 by LS

impurities

≤1% water

refractive index

n20/D 1.5290

viscosity

13,000-18,000(50 °C)

bp

250 °C (lit.)

density

1.030 g/mL at 25 °C

SMILES string

C1CN1

InChI

1S/C2H5N/c1-2-3-1/h3H,1-2H2

InChI key

NOWKCMXCCJGMRR-UHFFFAOYSA-N

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General description

Polyethylenimine (PEI) is a hydrophilic cationic polymer widely used as a nonviral nucleotide delivery reagent. Branched PEI can be synthesized by cationic ring-opening polymerization of aziridine. PEI-based particles can also be used as adjuvants for vaccines. Owing to its excellent physicochemical properties, it is applied in many fields like the separation and purification of proteins, carbon dioxide absorption, drug carriers, effluent treatment, and biological labels.

Application

Polyethyleneimine can be used as a non-viral synthetic polymer vector for in vivo delivery of therapeutic nucleic acids. The interaction between negatively charged nucleic acids and positively charged polymer backbone results in the formation of nano-sized complexes. This neutralized complex protects the enclosed nucleic acid from enzymes and maintains its stability till the cellular uptake takes place. For example, human serum albumin conjugated PEI shows good pDNA transfection and low toxicity.

PEI can be used to functionalize single-walled nanotubes (SWNTs) to improve their solubility and biocompatibility while maintaining the structural integrity of the original SWNT. Covalently functionalized SWNTs find application in CO2 absorption and gene delivery.

Branched PEI can also be used to modify the surface properties of adsorbents. PEI-modified hydrous zirconium oxide/PAN nanofibers are used for the defluorination of groundwater as they show high fluoride adsorption capacity and a wide working pH range.

Features and Benefits

Primary and secondary amine groups of PEI can efficiently bind to drugs, nucleic acids, and other functional moieties.

Branched PEI has better complexation andbuffering capacity.

Physical form

Branched polymer

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H302,H317,H319,H411

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM5483
BCCM5482
BCCM2321
STBL4117
STBL2116

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Jessel, N., et al.
Polym. Mater. Sci. Eng., 90, 755-755 (2004)
Yi-Hsuan Lee et al.
Acta biomaterialia, 63, 210-226 (2017-09-14)
The development of osteochondral tissue engineering is an important issue for the treatment of traumatic injury or aging associated joint disease. However, the different compositions and mechanical properties of cartilage and subchondral bone show the complexity of this tissue interface
Wei Dong et al.
Frontiers in immunology, 9, 2312-2312 (2018-10-26)
Sequential infection with antigenically distinct influenza viruses induces cross-protective immune responses against heterologous virus strains in animal models. Here we investigated whether sequential immunization with antigenically distinct influenza vaccines can also provide cross-protection. To this end, we compared immune responses
Hsuan-Wen Chiu et al.
Oncotarget, 8(55), 94129-94141 (2017-12-08)
The betanodavirus B2 protein targets the mitochondria and acts as a "death factor", but its effect on lung cancer cells is unknown. We examined the effect of the B2 protein on triggering apoptosis or necroptosis
Baoshun Lin et al.
Medicine, 94(31), e1301-e1301 (2015-08-08)
As an immunotoxin, diphtheria toxin has been widely used in gene therapy and gene function assays for its roles in protein synthesis inhibition, and the aim of our study is to set up a nonintegrating lentiviral system for specific expression
View All Related Papers

Articles

Functionalized PEI and Its Role in Gene Therapy

Gene therapy has become one of the most discussed techniques in biomedical research in recent years.

Nanoparticle-Based Drug and Gene Delivery for Tumor Targeting

Professor Yoshiki Katayama (Kyushu University, Japan) discusses recent advances in drug delivery systems and strategies that exploit the EPR effect, with a special focus on stimuli-responsive systems based on novel materials.

Progress on Nonviral Delivery for CRISPR/Cas9

CRISPR/Cas9 delivery via nonviral nanoparticles shows promising advancements for gene editing in disease treatment.

Polyelectrolyte Multilayer Films and Membrane Functionalization

We present an article that discusses two applications in particular; first, using these layers as polyelectrolyte membranes to control permeability.

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Questions

1–10 of 23 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Do you have any GPC (Gel permeation chromatography) data for this product n°408727 that you can share?

    1 answer

    1. This product is not tested by Gel Permeation Chromatography. The molecular weight distribution of this material has not been determined. However, historical information indicates the mole fraction of primary, secondary, and tertiary amines is 1:1.20:0.76. The material dispersity (Mw / Mn) is 25,000 / 10,000 = 2.5.

      Helpful?

  4. Is Branched PEI soluble in Diethylene glycol and other glycols? Do these solvents affect the amine functions/charges?

    1 answer

    1. This compound is readily soluble in hot water and some organic solvents, such as methanol, ethanol, or chloroform. However, the solubility in diethylene glycol or other glycols has not been determined.

      Helpful?

  5. Can this product be used for stable transfection?

    1 answer

    1. This product is not transfection grade and has not been tested as suitable for this type of application. It is the responsibility of the end user to determine suitability for this type of application. A search of the scientific literature does reveal that a few laboratories have reported the use of this product for transfection. Those article citations can be found below.

      Hoenigsperger, H., Koepke, L., Acharya, D., Hunszinger, V., Freisem, D., Grenzner, A., … Sparrer, K. M. J. (2023). CSNK2 suppresses autophagy by activating FLN-NHL-containing TRIM proteins. Autophagy, 1–21. https://doi.org/10.1080/15548627.2023.2281128

      Kritika Saxena, Nicola Domenico Roverato, Melody Reithmann, Mei Min Mah, Richard Schregle, Gunter Schmidtke, Ivan Silbern, Henning Urlaub, Annette Aichem. (2023) Phosphorylated FAT10 interferes with IFN-I signaling Life Science Alliance DOI: 10.26508/lsa.202101282

      Kirman, D.C., Renganathan, B., Chui, W.K. et al. Cell surface nucleolin is a novel ADAMTS5 receptor mediating endothelial cell apoptosis. Cell Death Dis 13, 172 (2022). https://doi.org/10.1038/s41419-022-04618-x

      Helpful?

  6. What recommended storage conditions (temperature) of PEI? Is refrigeration or freezing suitable? And how shelf-life at storage conditions? Impact of dilution on PEI's shelf-life ?

    1 answer

    1. Product no. 408727 Polyethylenimine, branched is stored at ambient temperature and does not have an assigned shelf-life.

      We offered a 2 mg/ml Branched polyethylenimine solution (Product no. 913375) that was stable for 2 years at 2 - 8 degrees C.

      Helpful?

  7. Can you please provide me with the number of primary amines per molecule for the product with the code 408727?

    1 answer

    1. Approximately 6.4 mmol of primary amines per gram of dry substance. This calculation is derived from the equation 1/(1+1.1+0.7) multiplied by 18, resulting in 6.4.

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  8. Is this polymer soluble in THF?

    1 answer

    1. The solubility of this product is not tested on a lot-to-lot basis. This compound is readily soluble in water and sparingly soluble in organic solvents such as DMSO and acetone. Other sources report that this material may also be sparingly soluble in THF. This information has not been validated.

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  9. What is the N(Nitrogen)% for following PEI? Polyethylenimine, branched, average Mw ~25,000 by LS, average Mn ~10,000 by GPC, branched, 250ml

    1 answer

    1. Unfortunately, the N content of Product 408727 is not determined.

      Helpful?

  10. What is the concentration of 408727-100ML in mg/ml?

    1 answer

    1. This product is a 'neat' or pure liquid. The density is 1.03 g/ml or 1030 mg/ml. The molarity of the material is 41.2 mM, based on average Mw ~25,000 by LS and the previously mentioned density.

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1–10 of 23 Questions  

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