If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
T4415
Tris-Borate-EDTA buffer
BioReagent, suitable for electrophoresis, 10× concentrate
Synonym(s):
TBE buffer
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 L | Available to ship TODAYfromMILWAUKEE | $77.20 | |
| 4 L | Available to ship TODAYfromMILWAUKEE | $175.00 | |
| 10 L | Available to ship TODAYfromMILWAUKEE | $389.00 $272.30 | |
| 20 L | Available to ship TODAYfromMILWAUKEE | $602.00 |
About This Item
Quality Level
product line
BioReagent
form
solution
impurities
DNase and RNase, none detected
suitability
suitable for electrophoresis
SMILES string
OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
InChI key
OSBLTNPMIGYQGY-UHFFFAOYSA-N
Looking for similar products? Visit Product Comparison Guide
Related Categories
1 of 1
This Item | |||
|---|---|---|---|
| product line BioReagent | product line - | product line pHast Pack™ | product line BioReagent |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 200 |
| form solution | form solution | form powder | form powder blend |
| suitability suitable for electrophoresis | suitability - | suitability suitable for gel electrophoresis | suitability suitable for electrophoresis |
| impurities DNase and RNase, none detected | impurities DNase and RNase, none detected | impurities - | impurities DNase and RNase, none detected |
General description
Application
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Tris-Borate-EDTA buffer has been used for pulsed-field gel electrophoresis (PFGE).
Packaging
Preparation Note
Other Notes
signalword
Danger
hcodes
Hazard Classifications
Repr. 1B
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Protocols
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| T4415-10L | 04061838260239 |
| T4415-20L | 04061838101280 |
| T4415-1L | 04061838260246 |
| T4415-4L | 04061838260253 |
-
How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service