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P8125

Sigma-Aldrich

Peroxidase from horseradish

Type I, essentially salt-free, lyophilized powder, ≥50 units/mg solid (using pyrogallol)

Synonym(s):

Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase

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$75.10
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$341.00
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$608.00
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$1,060.00

$75.10

Estimated to ship onFebruary 18, 2025Details

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5000 UNITS
$75.10
25000 UNITS
$193.00
50000 UNITS
$341.00
100000 UNITS
$608.00
200000 UNITS
$1,060.00

About This Item

CAS Number:
9003-99-0
Enzyme Commission number:
1.11.1.7 (BRENDA, IUBMB)
EC Number:
232-668-6
MDL number:
MFCD00071339
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

$75.10

Estimated to ship onFebruary 18, 2025Details

type

Type I

Quality Level

200

form

essentially salt-free, lyophilized powder

specific activity

≥50 units/mg solid (using pyrogallol)

mol wt

~44 kDa

solubility

0.1 M phosphate buffer: soluble (pH 6.0)
H2O: soluble

absorbance ratio

RZ ≥1.0

storage temp.

2-8°C

SMILES string

[O+H2]O[O-]

InChI

1S/H2O3/c1-3-2/h1-2H

InChI key

JSPLKZUTYZBBKA-UHFFFAOYSA-N

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General description

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

Application

Peroxidase from horseradish has been used:
  • in fecal starch analysis[1]
  • to measure plasma glucose levels by Trinder method[2]
  • to incubate feruloylated arabinoxylan fractions and also to evaluate oxidative coupling[3]
  • in the detection of glycoproteins[4]

Biochem/physiol Actions

HRP readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies by several different methods including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed cross-linkers. It is smaller and more stable than the enzyme labels β-galactosidase and alkaline phosphatase.Hence, it is the most desired label. Also, its glycosylation leads to lower non-specific binding.[5] It is also used for the determination of glucose[6] and peroxides[7] in solution. Sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, Pb2+ ions are known to inhibit the enzyme activity.[8]
When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule, allowing quantification.

Unit Definition

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Analysis Note

The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.

Other Notes

View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer.

inhibitor

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Description
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substrate

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Description
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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000400318
0000400317
0000400309
0000392090
0000385104

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Eva-Maria Herrlinger et al.
Chembiochem : a European journal of chemical biology, 21(16), 2329-2347 (2020-04-01)
Lysine-specific demethylase 1 (LSD1) has evolved as a promising therapeutic target for cancer treatment, especially in acute myeloid leukaemia (AML). To approach the challenge of site-specific LSD1 inhibition, we developed an enzyme-prodrug system with the bacterial nitroreductase NfsB (NTR) that
Apparent restriction of starch swelling in cooked noodles by lipids in some commercial wheat flours.
Kim, W. S., and P. A. Seib
Cereal Chem., 70(4), 367-372Kim-367-372Kim (1993)
Acarbose enhances human colonic butyrate production
Weaver G A, et al.
The Journal of Nutrition, 127(5), 717-723 (1997)
MBX-102/JNJ39659100, a Novel Non-TZD Selective Partial PPAR-gamma Agonist Lowers Triglyceride Independently of PPAR-alpha Activation
Chandalia A, et al.
PPAR Research, 2009 (2009)
K C Ngai et al.
Pediatric research, 45(4 Pt 1), 526-530 (1999-04-15)
Clinical observations suggest that sepsis may enhance the risk of kernicterus. This study investigated the combined effects of bilirubin, endotoxin, and tumor necrosis factor-alpha (TNF-alpha), which simulate sepsis in a jaundiced mouse fibroblast cell line. The horseradish peroxidase oxidation method
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Articles

Horseradish Peroxidase (HRP) Enzymes

Discover our peroxidase from horseradish enzymes, products, substrates, and inhibitors for your ELISA, immunoassay, and protein application needs.

Protocols

Spectrophotometric Determination of Reinheitszahl (RZ) for Peroxidase (EC 1.11.1.7)

Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

Enzymatic Assay of Peroxidase (EC 1.11.1.7)

This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.

Questions

  1. Hello! I am hoping to purchase this horseradish peroxidase to activate catharanthine in a reaction assay. How does the specific activity (pyrogallol units) translate to other molecules? And how should I calculate the amount of HRP to add to the reaction?

    1 answer

    1. Unfortunately, this product has not been tested for use in catharanthine activation. The end user would have to determine the appropriate amount experimentally. Please see the link below to review the product datasheet for additional information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/259/166/p8125dat-ms.pdf

      Please see the link below to review a publication that may be helpful:
      https://www.sciencedirect.com/science/article/pii/S0141022997000677

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