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NA2010

GenElute™ Blood Genomic DNA Kit

sufficient for 70 purifications

Synonym(s):

Blood Genomic DNA, Gen Elute

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1 kit

Available to ship TODAYfromMILWAUKEE

$237.00

About This Item

NACRES:
NA.55
UNSPSC Code:
41105501

$237.00

Available to ship TODAYDetails

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usage

sufficient for 70 purifications

Quality Level

200

technique(s)

DNA purification: suitable

input

blood

test parameters

: <40 min kit run time, sample volume: 200 μL

storage temp.

15-25°C, room temp

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GenElute&#8482; Blood Genomic DNA Kit sufficient for 70&#160;purifications

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GenElute&#8482; Blood Genomic DNA Kit sufficient for 350&#160;purifications

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GenElute&#8482; Mammalian Genomic DNA Miniprep Kits sufficient for 10&#160;purifications

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GenElute™ Mammalian Genomic DNA Miniprep Kits

GenElute&#8482; Bacterial Genomic DNA Kits sufficient for 10&#160;purifications

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technique(s)

DNA purification: suitable

technique(s)

DNA purification: suitable

technique(s)

-

technique(s)

-

usage

sufficient for 70 purifications

usage

sufficient for 350 purifications

usage

sufficient for 10 purifications

usage

sufficient for 10 purifications

BLD9601

BLD9601, BLD9604, NA2010

G1N350, G1N70, G1N9602, G1N9604

NA2110, NA2120

storage temp.

15-25°C

storage temp.

15-25°C, room temp

storage temp.

15-25°C

storage temp.

15-25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Click here to view the kit protocol
The GenElute Blood Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silica binding with a microspin format, and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Application

GenElute™ Blood Genomic DNA Kit has been used:
  • to extract DNA from samples of large granules (LG) and smaller granules (SG) obtained from vasectomized rabbits[1]
  • to isolate DNA from the blood samples [2]
  • to extract whole blood DNA from the left blood cells

The purified genomic DNA is ready for downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • sequencing reactions
  • cloning

Biochem/physiol Actions

The starting material is lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. A Prewash Solution is provided to help remove contaminants that are associated with aged (older than 24 hours) whole blood samples. After washing to remove contaminants, the DNA is eluted in 200 mL of a Tris-EDTA solution.

The expected yields of genomic DNA will vary depending on the amount and nature of the starting material used (for example, 4 to 10 μg of RNase A-treated DNA can be isolated from 200 μl of fresh whole blood in less than one hour). DNA purified with this kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Features and Benefits

  • Starting material: Up to 200 μl of fresh or aged blood
  • Expected yield: Up to 10 μg
  • Elution volume: 400 μl
  • Time required: <40 min
  • A260/A280 ratio: 1.6 - 1.9
  • Compatible with many anticoagulants, including EDTA, Heparin, and Sodium Citrate
  • Typical yields 4-10 μg from 200 μL of whole blood
  • Pure genomic DNA with a A260/A280 ratio between 1.6 and 1.9
  • Isolate DNA up to 50 kb in length

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS
  • P2308Proteinase K from Tritirachium album, lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein 3SDS
  • C2112Column Preparation SolutionSDS
  • R6148RNase A solutionSDS

related product

Product No.
Description
Pricing

P2308

Proteinase K from Tritirachium album, lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein

E1510

Ethidium bromide solution, BioReagent, Molecular Biology, 10 mg/mL in H2O

A9539

Agarose, BioReagent, Molecular Biology, low EEO

W4502

Water, Nuclease-Free Water, for Molecular Biology

NA2020

GenElute™ Blood Genomic DNA Kit, sufficient for 350 purifications

459836

Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%

L3287

Escort™ IV Transfection Reagent, Lipid reagent for transient and stable transfection of mammalian and insect cells.

T9661

Eppendorf® Safe-Lock microcentrifuge tubes, volume 1.5 mL, natural

T6400

Tris-Borate-EDTA buffer, 5× Concentrate

D9780

Lambda DNA Hind III Digest, for DNA electrophoresis

R6148

RNase A solution

C2112

Column Preparation Solution

93221-001

RNAstable®, Tube Kit, for room temp preservation of RNA

T0956

Sigma Universal Transfection Reagent, Polymer reagent for transfecting common mammalian cell lines

signalword

Danger

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

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Certificates of Analysis (COA)

Lot/Batch Number
0000532042
0000532042
0000463953
0000463953
0000463905

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Yanxia Gao et al.
Renal failure, 36(8), 1226-1232 (2014-07-01)
The objective of this study is to identify ATP6V1B1, ATP6V0A4 and SLC4A1 genes mutations and assess audiologic characteristics in six Chinese children with primary distal renal tubular acidosis from four unrelated families between the ages of 2 and 13 years.
Xiangzhong Zhao et al.
Scientific reports, 6, 33920-33920 (2016-09-27)
Familial renal glycosuria (FRG) is caused by mutations in the SLC5A2 gene, which codes for Na+-glucose co-transporters 2 (SGLT2). The aim of this study was to analyze and identify the mutations in 16 patients from 8 families with FRG. All
Cui Wang et al.
Scientific reports, 6, 33652-33652 (2016-09-21)
Primary hyperoxaluria type 1 (PH1) is a rare genetic disease characterized by excessive oxalate accumulation in plasma and urine, resulting in various phenotypes because of allelic and clinical heterogeneity. This study aimed to detect disease-associated genetic mutations in three PH1
Sai Wang et al.
Frontiers in genetics, 11, 585064-585064 (2020-12-01)
Familial renal glucosuria is a rare renal tubular disorder caused by SLC5A2 gene variants. Most of them are exonic variants and have been classified as missense variants. However, there is growing evidence that some of these variants can be detrimental
Yue Han et al.
Oncotarget, 8(60), 101614-101622 (2017-12-20)
Bartter syndrome (BS) has been rarely reported in Chinese population except for a few case reports. This investigation was aimed to analyze the mutations of the causal genes in sixteen Chinese patients with BS, and review their followup and treatment.
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Protocols

Blood Card - WGA Protocol

Protocol extracts genomic DNA from blood cards, useful for limited DNA samples in amplification.

Whole Genome Amplification from Serum or Plasma Protocol

Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.

GenElute™ Blood Genomic DNA Kit Protocol

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

See All

Global Trade Item Number

SKUGTIN
NA2010-1KT04061835573721

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