Based on previous results, a 20% difference in methylation is typically observable. It is recommended to conduct replicate experiments, assess the margin of error (technical and/or biological), and utilize a p-test or other statistical approach to evaluate its applicability in your specific case.
MDQ1
Imprint® Methylated DNA Quantification Kit
To measure global DNA methylation shifts from as low as 10 ng DNA
Synonym(s):
DNA Methylation Quantification, DNA methylation shift
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About This Item
UNSPSC Code:
41116158
NACRES:
NA.54
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General description
Imprint™ Methylated DNA Quantification technology provides a rapid and reliable method to measure global DNA methylation shifts. The Imprint Methylated DNA Quantification Kit contains all reagents required to detect relative levels of methylated DNA. Up to 200 ng of purified DNA is bound to the wells of the assay strip. The methylated DNA is detected using the Capture and Detection antibodies, then quantified colorimetrically. The amount of methylated DNA present in the sample is proportional to the absorbance measured.
Application
Features and Benefits
- ELISA based format
- Procedure completes in 4 easy to follow steps
- All reagents supplied including control methylated DNA
- Detection limit is 5 ng of fully methylated DNA
- Input DNA may be as low as 10-200 ng
- Procedure completes in 3.5 hours
- 96 well format allows option of studying single samples or high-throughput studies
- Optimized antibody and reagents with high specificity to 5-mC; no cross-reactivity to unmethylated cytosine
- May be used with adherent and suspension cells, tissues, plasma and other body fluids such as saliva, urine and serum
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Kit Components Also Available Separately
Product No.
Description
SDS
- M8570Methylated Control DNA, 50 ng/μLSDS
related product
Product No.
Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Met. Corr. 1 - Skin Sens. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
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Articles
Global DNA Modification Kit (MDQ1) monitors DNA methylation via sandwich ELISA-like assay, aiding in epigenetic research.
Protocols
Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
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Could you please provide the sensitivity of the kit and explain what differences in methylation status it can detect?
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Are there any species restrictions for Product MDQ1, Imprint® Methylated DNA Quantification Kit?
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The antibody works on 5-me-C, so any species with DNA that is methylated at the 5 position of cytidine residues will work.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the detection limit of Product MDQ1, Imprint® Methylated DNA Quantification Kit?
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The detection limit of this kit is 5 nanograms of fully methylated DNA.
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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When using Product MDQ1, Imprint® Methylated DNA Quantification Kit, can I dilute the entire antibody at once and save it for later?
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No. A fresh dilution of the antibody should be prepared each time the kit is used. Diluting the antibody all at once will decrease its stability and it's effectiveness in the kit.
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Is Product MDQ1, Imprint® Methylated DNA Quantification Kit, shipped in two parts?
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Yes, Product MDQ1, Imprint® Methylated DNA Quantification Kit, is shipped in 2 parts. Part 1 contains, 2-8 °C components (W4267, D8568, C3493, D8818, D8693, S3447, and A4231) and Part 2 contains -20 °C components (M8570 and B6561).
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When using Product MDQ1, Imprint® Methylated DNA Quantification Kit, do I need to use all the wells in the plate at once?
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No, you can use as many wells as needed. You will want to ensure that all the wells that are not being used are covered well with Parafilm, Saran Wrap or some other plate sealer.
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Can I use chloroform to extract my DNA before using Product MDQ1, Imprint® Methylated DNA Quantification Kit?
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Choroform extraction should not interfere with the protocol as long as the DNA was not eluted with the chloroform.
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