Both frozen sections and paraffin-embedded tissue are considered satisfactory for iron staining, as per the Instructions For Use. Blood or bone marrow films are prepared in the usual fashion, air-dried for at least 30 minutes, and fixed in absolute methanol for 7 minutes.
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About This Item
UNSPSC Code:
41116121
NACRES:
NA.47
Recommended Products
form
solution
Quality Level
shelf life
Expiry date on the label.
IVD
for in vitro diagnostic use
application(s)
hematology
histology
storage temp.
room temp
Principle
Deposits of loosely bound iron in erythrocytes, bone marrow, or tissue stain an intense blue when treated with acid ferrocyanide according to the well-known Prussian blue reaction.
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Carc. 1B - Flam. Liq. 2 - Met. Corr. 1 - STOT SE 1
Target Organs
Eyes,Central nervous system
Storage Class Code
3 - Flammable liquids
Flash Point(F)
51.8 °F
Flash Point(C)
11 °C
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Related Content
Learn about the clinical study of blood, blood-forming organs, and blood diseases including the treatment, prevention, and stains and dyes used in hematology testing.
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Can the HT20 Iron Stain kit be used with frozen sections?
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Is ECM590 compatible with Prussian blue staining, and does it work for both paraffin and frozen sections?
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It's uncertain whether it would be possible to dual stain a tissue section with a Prussian Blue staining procedure and the IHC kit ECM590. However, it's recommended to try the ECM590 kit and then perform the Prussian Blue staining procedure. The color contrast should not be a problem, but the use of DAB for demonstrating both anti-von Willebrand Factor and anti-CD31 primary antibodies may lead to brown staining for both antibodies.
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Does EDTA affect Prussian Blue staining for siderocytes and other blood cells found in bone marrow?
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While slides anticoagulated with EDTA may be suitable for demonstrating iron in peripheral blood smears and bone marrow smears, bone marrow that has been decalcified in EDTA may not demonstrate iron using the Perl’s or Prussian Blue Method. Even for bone marrow smears, EDTA can diminish or remove iron from bone marrow aspirates if the levels of EDTA used in collection are high. EDTA has various uses in laboratories, including as an anticoagulant and a decalcifying agent to chelate calcium from bone. By removing the calcium, bone can be processed into paraffin blocks and cut into thin sections using a microtome. EDTA can also be used to chelate a number of other metals, including iron. Once the iron deposits have been removed from osseous bone, the iron deposits are also removed from cells at the same time. Consequently, bone marrow smears that have been decalcified in EDTA will generally stain negative for iron in bone marrow slides.
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Is it necessary to use the counterstain to view tissue Iron when using the Iron Stain Kit, Product HT20?
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Iron in tissue samples (used for research) can be visualized without the Pararosaniline counterstain, which is used to allow improved visualization of the tissue.
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Can the Iron Stain Kit, Product HT20, be used to stain cultured cells, rather than blood or bone marrow?
1 answer-
This kit can be used to stain cultured mammalian cells, which should be treated as blood or bone marrow and follow instructions given for specimen collection in the Package Insert. We advise using some sort of positive control.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is EDTA an acceptable anticoagulant for use with the Iron Stain Kit, Product HT20?
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Blood and bone marrow samples should be drawn without the use of EDTA as an anticoagulant. The EDTA chelates enough Iron to interfere with adequate staining. We advise using heparinized blood or freshly drawn blood which has not been anticoagulated.
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Can I buy the reagents in Iron Stain Kit, Product HT20, separately?
1 answer-
The reagents included in this kit are not available for separate purchase.
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