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G6392
L-Glutamine
γ-irradiated, BioXtra, suitable for cell culture
Synonym(s):
(S)-2,5-Diamino-5-oxopentanoic acid, L-Glutamic acid 5-amide, Levoglutamide
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 vial | Estimated to ship onMay 11, 2026fromMILWAUKEE | $28.30 | |
| 10 vials | Available to ship TODAYfromMILWAUKEE | $291.00 |
About This Item
Linear Formula:
H2NCOCH2CH2CH(NH2)CO2H
CAS Number:
Molecular Weight:
146.14
MDL number:
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
EC Number:
200-292-1
NACRES:
NA.75
Beilstein/REAXYS Number:
1723797
Quality Level
sterility
γ-irradiated
product line
BioXtra
form
powder
packaging
vial of 0.292 g
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
white to faint yellow
mp
185 °C (dec.) (lit.)
solubility
H2O: 10 mL/vial, clear to slightly hazy
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This Item | |||
|---|---|---|---|
| form powder | form powder | form solid | form powder |
| product line BioXtra | product line - | product line - | product line BioUltra |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable |
| color white to faint yellow | color white | color - | color white |
| impurities endotoxin, tested | impurities endotoxin, residual solvents, tested | impurities - | impurities insoluble matter, passes filter test, ≤0.5% foreign amino acids |
| solubility H2O: 10 mL/vial, clear to slightly hazy | solubility H2O: 25 mg/mL | solubility 100 g/L | solubility H2O: 0.1 M at 20 °C, clear, colorless |
Application
L-Glutamine has been used as a media supplement:
- for cochlear organotypic culturing
- in Iscove′s modified Dulbecco′s medium for HeLa cells culturing
- in Dulbecco′s modified Eagle′s medium for primary hepatocyte cell culturing
Qualified for use in cell culture applications, especially serum-free cell culture.
Biochem/physiol Actions
Glutamine mediates protein turnover via cellular mTOR (mammalian target of rapamycin) signalling. It is also known to be associated with the inhibition of apoptosis.
L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.
L-glutamine is an essential amino acid that is a crucial component of culture media, and serves as a major energy source for cells in culture. Optimal cell performance usually requires supplementation of the medium with L-glutamine prior to use.
Packaging
50 mL amber serum vial with rubber stopper.
Preparation Note
Prepares a 200 mM solution when reconstituted to 10 mL with sterile water.
Other Notes
The dipeptide L-Ala-LGln is frequently used in modern serum-free media formulations because it provides a more stable form of glutamine in vitro.
related product
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Articles
Importance and uses of glutamine in hybridoma and mammalian cell culture
Protocols
Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.
WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.
MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.
Protocolo del ensayo con MTT para medir la viabilidad, la proliferación y la citotoxicidad celulares. Instrucciones para la preparación de los reactivos MTT y ejemplos de aplicaciones.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G6392-1VL | 04061833639122 |
| G6392-10VL | 04061833639115 |
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What is the Department of Transportation shipping information for this product?
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Is the molecular weight of L-Glutamine 146.14 or 146.15? The label information changed.
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Based on a molecular formula of C5H10N2O3, the molecular weight to seven significant figures is 146.1456. Over the years, this value may have been reported in Sigma-Aldrich documentation or on labels as either 146.15 (by rounding up) or 146.14 (by truncation). The material itself has not changed.
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