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G1393

Sigma-Aldrich

Bovine Gelatin

from bovine hide, Type B, liquid, suitable for cell culture

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About This Item

CAS Number:
9000-70-8
MDL number:
MFCD00081638
UNSPSC Code:
12352202
NACRES:
NA.75

product name

Gelatin solution, Type B, 2% in H2O, tissue culture grade, BioReagent, suitable for cell culture

biological source

bovine hide

Quality Level

200

sterility

sterile

type

Type B

product line

BioReagent

form

solution

packaging

pkg of 100 mL
pkg of 20 mL

concentration

2% in H2O, tissue culture grade

technique(s)

cell culture | mammalian: suitable

surface coverage

100‑200 μg/cm2

impurities

endotoxin, tested

solubility

water: soluble

binding specificity

Peptide Source: Fibrinogen

shipped in

ambient

storage temp.

2-8°C

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General description

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue. This is a hydrocolloid and is rich in glycine, proline and hydroxyproline, which imparts structural stability. It is synthesized from the alkaline digestion of collagen from bovine skin and is referred as type B. Gelatin has wide applications in food industry. It takes up random coil structure after digestion from the triple helical collagen. The most common source for industrial production of gelatin is slaughter byproducts. The type A gelatin and type B differ in their isoelectric pH. This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objective.

Application

Gelatin solution has been used:
  • for coating of cell culture plates or dishes used for embryonic stem cell culture, testicular cell culture and neural rosettes
  • in coating cell culture to improve attachment of cells
  • in PCR to stabilize Taq DNA
  • as a blocking reagent in western blotting
  • in ELISA
  • in immunochemistry
  • as a component of media for species differentiation in bacteriology
  • to study long-chain fatty acid-induced changes in gene expression in neonatal cardiac monocytes
  • to study to test mobilization of capillary endothelium in vitro induced by effectors of angiogenesis in vivo

Biochem/physiol Actions

Gelatin has gelling property and displays surface behaviour for use in foams and adhesions. It also serves as a clarification agent in fruit based juices. It is a digestible protein and serves as a binding agent, thickener, stabilizer and emulsifier in food industry. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Preparation Note

This product is derived lime-cured tissue bovine skin, and is a 2% solution in water.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The TGFbeta pathway is a key player for the endothelial-to-hematopoietic transition in the embryonic aorta
Lempereur A, et al.
Developmental Biology, 434(2), 292-303 (2018)
Gelatin, source, extraction and industrial applications
Mariod AA and Fadul H
Acta Scientiarum Polonorum. Technologia Alimentaria, 12(2), 135-147 (2013)
Mutations in LRRK2 impair NF-kappaB pathway in iPSC-derived neurons
de Maturana RL, et al.
Journal of Neuroinflammation, 13(1), 295-295 (2016)
Optimal culture conditions are critical for efficient expansion of human testicular somatic and germ cells in vitro
Gat I, et al.
Fertility and Sterility, 107(3), 595-605 (2017)
Pedro Barbacena et al.
Genesis (New York, N.Y. : 2000), 57(6), e23299-e23299 (2019-04-17)
Cell migration is essential during development, regeneration, homeostasis, and disease. Depending on the microenvironment, cells use different mechanisms to migrate. Yet, all modes of migration require the establishment of an intracellular front-rear polarity axis for directional movement. Although front-rear polarity
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