Primary Cell Culture
Primary cells are derived directly from living tissue, providing researchers with more meaningful models of human health and disease and more closely replicating the physiology of biological systems. They are increasingly useful in life science research, ADME/toxicity studies, and pharmaceutical development, and in diverse applications where cell lines do not replicate the target biological system or where immortalized cell lines have attributes that can confound results.
Our collection of primary cells comes with the promise of purity, low passage, rigorous characterization, and strict quality control. Harmonized media and reagents provide optimal performance, ensuring confidence in the utility of these cells and their supporting products in your application. We offer PromoCell® human primary cells (available in select geographies), primary blood and immune cells from BioIVT, and primary cells from Cell Applications, Inc. (CAI).
Section Overview
PROMOCELL® Human Primary Cell Culture
PromoCell® products are available in North America, Canada, and select countries in Europe and Asia.
Our primary cell collection includes cells and precisely formulated media from industry-leading manufacturer PromoCell®. We offer a broad range of validated, ethically-sourced human primary and stem cells, plus human blood cells—along with culture media optimized for expert culture of these phenotypes.
- Authenticated primary cells from dozens of human tissue sources, with media formulated for successful culture
- Expertly-crafted primary cell culture protocols, including for tumor spheroid formation, culturing human cardiac myocytes, generating organotypic air-liquid interface (ALI) 3D skin cultures, and more
- Advanced media and reagents for 3D culture from patient cancers
- Ethically collected human primary cells with donor documentation, and validated for identity with definitive phenotypic markers
Unique formulations for groundbreaking cancer studies include the Primary Cancer Culture System, a complete solution for the selective culture of malignant cells extracted from primary tumors or patient xenografts.
BioIVT peripheral blood mononuclear cells (PBMCs) and immune cell subsets
In partnership with BioIVT, we offer an extensive collection of ready-to-use normal and disease state PBMCs as well as a variety of pre-isolated immune cell subsets with guaranteed purity, sterility, and yield. Our PMBCs and immune cells can be stored in the freezer until needed, with a demonstrated viability and functionality.
- Wide range of cryopreserved PBMCs and immune cell subtypes including B cells, T cells, and NK cells for applications such as ADCC and T cell killing assays, drug toxicity assays, and more.
- HLA typing available for many cell types
- Request specific donor information to meet study-specific inclusion and exclusion donor criteria
Get answers to your questions about PMBCs and immune cells with our frequently asked questions technical article.
Click Here to Request Information about specific donor demographics or other criteria.
Cell Applications Inc. Primary Cells
Cell Applications Inc. has perfected the isolation, purification, subculture and growth of human and animal primary cells. Diverse primary cell options include cryopreserved formats, and cells prescreened for metabolic regulation markers and signaling pathways.
Diverse primary cell options include cryopreserved formats, and cells prescreened for metabolic regulation markers and signaling pathways.
Related Product Resources
- /deepweb/assets/sigmaaldrich/marketing/global/documents/212/514/promocell-br6025en-ms.pdf
- Primary Cell Culture FAQ
Primary Cell Culture: Frequently Asked Questions (FAQs)
- Introduction to Primary Cell Culture
Yeast plasmid selection: Utilizes auxotrophic yeast strains for plasmid selection, enabling growth on synthetic media for better yield.
- Primary Cell Culture Basics
Overview of primary cells and media usage in biomedical culture applications highlights differences from cell lines.
- Generating Organotypic Air-Liquid Interface (ALI) 3D Skin Cultures as an In-Vitro Model of the Human Epidermis
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
- Primary Human Chondrocytes and Chondrocyte Growth Media
Primary human chondrocyte cells and optimized growth media for the in vitro cultivation of chondrocytes. Protocols for handling, cell passaging, media, and product use.
- Primary Human Fibroblasts and Growth Media
Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.
- Tumor Spheroid Formation Assay
3D cell culture protocol: the tumor spheroid formation assay using a serum-free and xeno-free cancer stem cell culture media.
- Primary Human Endothelial Cells and Growth Media
Primary human endothelial cells isolated from microvascular tissue plus optimized growth media, with handling and protocol details.
- Primary Human Mononuclear Cells Culture
Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.
- Primary Airway Epithelial Cells
Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.
- Primary Human Mononuclear Cells Culture
Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.
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